诊断唾液样本中的遗传多样性反映在Mycobacterium结核病菌在不同环境中的培养中
Carla Mariner-Llicer1, Galo A Goig2,3, Manuela Torres-Puente1
1Instituto de Biomedicina de Valencia, IBV, CSIC, València, Spain.
Nature communications
|September 5, 2024
概括
基于培养的测序准确地反映了临床样本中的Mycobacterium结核病 (MTB) 基因组,挑战了多样性瓶的概念. 这一发现对于结核病 (TB) 研究和诊断至关重要.
科学领域:
- 基因组学就是基因组学.
- 微生物学 微生物学
- 传染性疾病 传染性疾病
背景情况:
- 结核菌菌菌 (MTB) 的培养和基因组测序是结核病 (TB) 研究和临床应用的标准.
- 从诊断样本中进行无培养测序提供了一个替代方案,但也带来了重大的分析挑战.
- 矛盾的是,人们认为文化给MTB带来了多样性瓶,其后果尚不清楚.
研究的目的:
- 研究培养对MTB基因组多样性的影响.
- 开发和验证一种工作流程,用于从唾液样本中进行无培养测序.
- 为了比较配对的唾液和培养样本的基因组数据,以解决差异.
主要方法:
- 在多种不同的环境中从唾液培养对生成高质量的MTB基因组.
- 开发了一种专门的生物信息学管道,用于分析液中的MTB基因组.
- 进行了详细的比较分析,以识别和解释变化不一致的来源.
主要成果:
- 在唾液培养比较中确定了不一致的来源,包括低DNA输入和可变的测序深度.
- 在考虑了技术因素后,在唾液和培养衍生MTB基因组之间观察到高变异一致 (97%).
- 变异频率也显示了配对样本之间的强相关性 (0.98).
结论:
- 与瓶假说相反,MTB培养精确地反映了临床唾液样本中存在的遗传多样性.
- 开发的测序和分析工作流程在不同的结核病流行情景中是强大的.
- 这些发现验证了在结核病诊断和研究中使用基于培养的测序来进行可靠的MTB基因组分析.
更多相关视频
07:42A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas
Published on: June 5, 2014
8.0K
11:23Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples
Published on: December 22, 2014
37.2K
相关概念视频
01:26Pulmonary Tuberculosis IV
134
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
134
01:26Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture
172
Sputum studies are a critical part of diagnosing and treating numerous respiratory conditions. These studies involve obtaining sputum samples for analysis to identify pathogenic organisms and assess the presence of abnormal cells indicative of malignant conditions. This lesson will delve into three fundamental sputum studies: Gram Stain, Cytology, and Acid-fast Smear and Culture.
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
172
01:20Sputum Studies II: Culture and Sensitivity
275
Description
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
275
01:29Pulmonary Tuberculosis I
227
Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
227