相关实验视频

Updated: Jun 14, 2025

Depletion and Reconstitution of Macrophages in Mice

Depletion and Reconstitution of Macrophages in Mice

Published on: August 1, 2012

由CAFs衍生出的异位体miR-889-3p可能抑制M1巨的极化,通过调节STAT1来促进ESCC的发展

Shaofeng Zhang¹, Danqing Li², Haijun Wang¹

¹Department of Thoracic surgery, Xingtai People's Hospital, Xingtai, China.

Cell biochemistry and biophysics

|September 5, 2024

概括

与癌症相关的纤维细胞通过外体转移microRNA-889-3p,通过抑制M1巨分化和下调STAT1.1,促进食道状细胞癌 (ESCC) 的进展. 这突出了miR-889-3p作为ESCC的潜在治疗点.

科学领域:

在瘤学瘤学.
细胞生物学细胞生物学
免疫学免疫学免疫学

背景情况:

与癌症相关的纤维细胞 (CAF) 有助于免疫抑制瘤微环境.
来自CAF的外体可能会促进食道状细胞癌 (ESCC) 的进展.
来自CAFs的外体微RNA-889-3p (miR-889-3p) 在ESCC中的特定作用仍然不清楚.

研究的目的:

调查CAFs衍生的外体 miR-889-3p在ESCC进展中的作用和机制.
为了确定CAFs衍生的外体miR-889-3p是否影响巨细胞两极分化.
阐明ESCC中CAF衍生出异位体miR-889-3p的分子标和体内影响.

主要方法:

使用电子显微镜和纳米粒子跟踪分析对外体的表征.
通过流细胞计和分子标记物 (TNF-α,iNOS) 评估巨细胞极化.
评估ESCC细胞的增殖,循环,迁移和入侵 (CCK-8,EdU,scratch,Transwell测定);蛋白质分析 (Western blot);体内异种移植模型;生物信息学预测和miR-889-3p标 (STAT1) 的实验验证.

主要成果:

由CAFs衍生出的异位体miR-889-3p抑制了M1巨细胞的两极分化,并在M0巨细胞中减少.
Knockdown 的外体 miR-889-3p 抑制 ESCC 细胞的增殖,迁移和入侵.
miR-889-3p直接针对STAT1,而CAFs衍生的外体 miR-889-3p通过降低STAT1的调节,在体内加速ESCC瘤的生长.

结论:

由CAFs衍生出的异位体miR-889-3p通过通过STAT1下调节抑制M1巨细胞极化来促进ESCC的进展.
这一途径代表了瘤微环境中的新机制.
来自CAFs的外体miR-889-3p是食道状细胞癌的潜在治疗标.

关键词:

这些CAF是CAF.在 ESCC ESCC 的位置上.巨细胞的两极分化美国国家统计局 (STAT1)在 miR-889-3p 中使用.

更多相关视频

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Published on: April 3, 2017

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

Published on: July 16, 2021

相关概念视频

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...