相关实验视频

Updated: Jun 14, 2025

Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis

Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis

Published on: November 8, 2024

AC092100.1通过YTHDC2 / VEGFA信号传递促进先兆子的血管生成

Wenjing Yong^1,2, Yu Jian^1,2, Qi Wang^1,2

¹Department of Obstetrics, Xiangya Hospital, Central South University, Changsha, Hunan, China.

Functional & integrative genomics

|September 5, 2024

概括

长非编码RNA AC092100.1 在妊娠前 (PE) 中是下调调节的. 上调AC092100.1通过与YTHDC2和VEGFA相互作用促进血管生成,为PE提供潜在的治疗点.

科学领域:

分子生物学分子生物学
生殖生物学生殖生物学
遗传学遗传学是一个

背景情况:

异常长非编码RNA (lncRNA) 表达涉及到先兆子 (PE) 的病原发生.
在PE中大多数lncRNAs的特定功能和机制仍然不太清楚.
研究像AC092100.1这样的新型lncRNA对于理解PE至关重要.

研究的目的:

在PE的背景下,研究lncRNA AC092100.1在血管生成中的调节作用.
阐明 AC092100.1 影响血管生成过程的分子机制.
评估针对PE中AC092100.1途径的治疗潜力.

主要方法:

生物信息学分析以确定PE患者中差异表达的lncRNAs.
定量逆转录PCR (qRT-PCR) 用于验证胎盘组织中的AC092100.1水平.
使用人类静脉内皮细胞 (HUVEC) 进行体外研究,以评估增殖,迁移和管形成.
RNA结合测试以确认AC092100.1和YTHDC2.2之间的相互作用.
使用PE小鼠模型进行VEGF-A表达分析和体内研究.

主要成果:

在PE患者的胎盘组织中,AC092100.1被发现显著下调.
过度表达AC092100.1增强了HUVEC的扩散,迁移和管道形成.
AC092100.1与YTHDC2结合,导致HUVEC中的YTHDC2和VEGFA积累增加.
抑制YTHDC2或VEGFA可以抵消AC092100.1.1.的益血管效应.
AC092100.1 在PE小鼠模型中的过度表达改善了胎儿生长和血管原生标记的正常化.

结论:

在血管生成的调节中,lncRNA AC092100.1 起着至关重要的作用.
AC092100.1/YTHDC2/VEGFA轴是一个关键的分子通路,涉及PE的病原性.
准AC092100.1通路是一个有前途的治疗策略.

关键词:

在AC092100.1上,您可以在AC092100.1上查看血管新生的产生.孕产妇产前症孕产妇产前症在VEGFA中,VEGFA是VEGFA.这就是YTHDC2

更多相关视频

Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay

Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay

Published on: June 30, 2023

In Vitro Model of Coronary Angiogenesis

In Vitro Model of Coronary Angiogenesis

Published on: March 10, 2020

相关概念视频

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

Mechanism of Angiogenesis

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...