Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

DNA Microarrays02:34

DNA Microarrays

17.2K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
17.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Epigenetic conditioning improves sequence-based modeling of gene regulation across cell types and alleles.

bioRxiv : the preprint server for biology·2026
Same author

Single-molecule variation in telomeric sequence and structure across humans.

bioRxiv : the preprint server for biology·2026
Same author

Haplotype-resolved centromeric chromatin organization from a complete diploid human genome.

bioRxiv : the preprint server for biology·2026
Same author

In vitro reconstitution of heterochromatin compartments reveals the spontaneous formation of tunable liquid-liquid interfaces.

Nucleic acids research·2026
Same author

Phosphorylation of Xenopus M18BP1 governs centromeric localization and CENP-A nucleosome assembly.

EMBO reports·2026
Same author

Author Correction: Heterochromatin boundaries maintain centromere position, size and number.

Nature structural & molecular biology·2026
Same journal

iMUT-seq mapping of DSB-induced mutations with high sensitivity at single-nucleotide resolution.

Nature protocols·2026
Same journal

An assay to quantify sexual commitment and stage conversion in the human malaria parasite Plasmodium falciparum.

Nature protocols·2026
Same journal

Author Correction: Direct inoculation of bioreactor-controlled stirred suspension culture with cryopreserved human pluripotent stem cells.

Nature protocols·2026
Same journal

High-throughput measurements of protein domain functions using magnetic separation.

Nature protocols·2026
Same journal

Inducing physiological polarity and performing gene editing using CRISPR-Cas9 in human trophoblast organoids.

Nature protocols·2026
Same journal

Photocatalytic low-temperature defluorination of PTFE.

Nature protocols·2026
查看所有相关文章

相关实验视频

Updated: Jun 14, 2025

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
09:14

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

Published on: January 27, 2016

19.4K

用DiMeLo-seqq绘制蛋白质-DNA相互作用的地图.

Annie Maslan1,2,3, Nicolas Altemose4,5, Jeremy Marcus3

  • 1Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.

Nature protocols
|September 5, 2024
PubMed
概括
此摘要是机器生成的。

使用长读序列 (DiMeLo-seq) 的定向甲基化提供了一种全基因组蛋白质-DNA相互作用映射的新方法. 这种技术准确地描述了相互作用,即使是在具有挑战性的基因组区域.

更多相关视频

Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae
10:43

Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae

Published on: June 3, 2017

11.1K
CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

6.7K

相关实验视频

Last Updated: Jun 14, 2025

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
09:14

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

Published on: January 27, 2016

19.4K
Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae
10:43

Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae

Published on: June 3, 2017

11.1K
CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

6.7K

科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 绘制蛋白质-DNA相互作用的地图对于理解基因组调节至关重要.
  • 现有的短读方法在重复区域和单元型分辨率方面存在局限性.
  • 在内源性DNA甲基化背景下,需要一种方法来描述蛋白质结合.

研究的目的:

  • 介绍指导甲基化与长读序列 (DiMeLo-seq) 的详细方案.
  • 为在各种细胞制剂上执行DiMeLo-seq提供实用指导.
  • 以增强的分辨率实现蛋白质-DNA相互作用的全基因组映射.

主要方法:

  • 在目标蛋白附近的腺基的in situ甲基化,使用绑定的Hia5甲基转移酶.
  • 通过长时间读取的单分子测序 (例如,纳米孔) 检测甲基化氨酸.
  • 使用A蛋白将抗体与Hia5甲基转移酶结合起来.

主要成果:

  • DiMeLo-seq使单个DNA分子上多个相互作用点的映射成为可能.
  • 在内源性DNA甲基化背景下描述蛋白质结合.
  • 识别单元型特定的蛋白质-DNA相互作用,并在重复的基因组区域映射相互作用.

结论:

  • DiMeLo-seq是一种用于全面绘制蛋白质-DNA相互作用的多功能方法.
  • 该协议可以适应新鲜,轻度固定或冷的细胞.
  • 有一个Python包 (dimelo) 可用于数据分析.