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不同的料摄入水平对小猪肠道形态和上皮细胞分化的影响

Xin Wang¹, Lanmei Yin¹, Chunchun Geng¹

¹Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China.

Journal of animal science

|September 6, 2024

概括

在小猪中限制料减少了肠道发育和细胞分化,除了杯状细胞. 介素-17和介素-22调节这些变化,影响整体肠道健康.

科学领域:

动物科学动物科学
胃肠病学胃肠病学
发展生物学发展生物学

背景情况:

料摄入对于断奶小猪的最佳肠道发育至关重要.
了解料限制对肠道形态和细胞分化的影响,对于动物健康和生产力至关重要.

研究的目的:

研究不同料摄入水平对小猪肠道形态和上皮细胞分化的影响.
探索这些变化背后的分子机制,包括细胞因子和途径参与.

主要方法:

64只小猪被分为四个料摄入组 (100%,80%,40%,20%) 并在3天和7天取样.
分析了肠道形态 (维卢斯高度,密室深度),细胞增殖 (Ki67) 和分化标志物.
用RT-qPCR和丰富分析进行了细胞因子 (IL-17,IL-22) 的基因表达和途径分析 (PPAR,ECM受体相互作用).

主要成果:

限制料显著降低了体重,器官指数,的高度和密室深度.
杰细胞增殖和分化标志物随着限制而下降,除了杯状细胞,其表现出复杂的变化.
IL-17和IL-22的表达增加,PPAR信号传递和ECM受体相互作用通路被激活,特别是在限制7天后.

结论:

增加料限制的强度和持续时间会对小猪肠道形态和上皮细胞的增殖和分化产生负面影响.
玻璃杯细胞反应是一个例外,表明一个不同的调节机制.
由Th细胞分泌的IL-17和IL-22在料限制下在调节肠道分化方面发挥作用.

关键词:

料摄入水平料摄入水平肠道上皮细胞是肠道上皮细胞肠道形态肠道形态肠道的增殖和分化.小猪小猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪

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