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高通量测序检测到一个单一的pokeweed植物中的病毒-viroid复合体

Myeonghwan Kwak¹, Elisa Troiano², Eui-Joon Kil¹

¹Department of Plant Medicals, Andong National University, Andong, Republic of Korea.

Frontiers in plant science

|September 6, 2024

概括

这项研究报告了意大利首次检测到黄病毒 (TuYV),该病毒在美国植物 (Phytolacca americana) 中发现. 鉴定出了一种新的复合TuYV分离物和一种花外皮病毒型变体,突出了杂草植物作为潜在的病毒储库.

科学领域:

植物病毒学植物病毒学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

美国植物 (Phytolacca americana),通常被称为pokeweed,是一种在植物疾病流行病学中具有潜在作用的杂草物种.
黄病毒 (TuYV) 和皮外皮病毒 (CEVd) 是影响各种作物的重要植物病原体.
了解病毒多样性和宿主相互作用对于疾病管理至关重要.

研究的目的:

为了表征与意大利一个有症状的美国植物Phytolacca相关的病毒和病毒病原体.
在TuYV分离物中调查遗传多样性和潜在的重组事件.
评估P. americana作为植物病毒和病毒病毒的潜在储库的作用.

主要方法:

总RNA高通量测序 (HTS) 用于全面的病毒和病毒病毒发现.
生物信息分析用于基因组组装和序列表征.
进行了重组分析,以确定潜在的遗传交换事件.

主要成果:

在P. americana中发现了两种新的TuYV分离体 (TuYV-ITA1和TuYV-ITA2) 和一种新的CEVd变体 (CEVd-ITA1).
TuYV-ITA2表现出基因变异性和与未识别的波列罗病毒重组的证据,特别是在P5读透域中.
这代表了TuYV在意大利的第一个报告,并建议P. americana作为TuYV和CEVd的新型自然宿主.

结论:

美国植物可以藏并可能传播TuYV和CEVd,这表明它作为病毒储存库的作用.
发现复合TuYV分离物的发现强调了植物病毒的动态进化.
需要进一步的研究来评估P. americana在TuYV传播中的流行病学意义及其对意大利农业的潜在威胁.

关键词:

在 CEVd 中.美国植物 (Phytolacca americana) 是一种波莱罗病毒病毒 (Polerovirus) 是一种病毒.在TuYVV中,你会看到.新兴病毒是新出现的病毒.下一代测序的下一代测序.

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