循环二合成酶的固定使生物催化剂能够用于循环二生产
Lynette Alvarado-Ramírez1, Emmajay Sutherland2, Elda M Melchor-Martínez1,3
1School of Engineering and Sciences, Tecnologico de Monterrey, Monterrey 64849, Mexico.
ACS sustainable chemistry & engineering
|September 6, 2024
概括
使用生物炭,酸盐或酸盐的酶固定支持显著增强循环二聚的生产和酶的可重复使用性. 这种方法支持复杂的酶反应,包括涉及tRNA的酶反应.
科学领域:
- 生物技术是生物技术.
- 酶工程是什么? 酶工程是什么?
- 自然产品的合成自然产品的合成
背景情况:
- 循环二合成酶 (CDPS) 生产有价值的循环二,但通常表现出不良的稳定性和可回收性.
- 酶固定是提高催化剂效率并使生物催化剂重复利用的关键策略.
研究的目的:
- 来自 *Parcubacteria细菌* RAAC4_OD1_1 的 CDPS 酶固定,以提高其稳定性和可回收性.
- 通过固定CDPS研究循环二聚循环 ((His-Glu) (cHE) 的产生,并探索联合固定策略.
主要方法:
- 在生物炭,酸和酸盐珠上固定 *Parcubacteria细菌* CDPS (PbCDPS).
- 使用高性能液体染色学-质谱学 (HPLC-MS) 确认cHE生产.
- 与histidyl-tRNA合成酶和谷氨基基-tRNA合成酶共同固定PbCDPS,用于级联反应.
主要成果:
- 在所有测试的支上成功固定了活跃的PbCDPS.
- 通过固定化的酶,可以实现cHE产量的5倍增加,这些酶在7个周期内保持活跃.
- 协同固定化的酶成功地产生了cHE,tRNAs在溶液中保持自由.
结论:
- 在生物炭,酸盐和酸盐支上的酶固定有效地增强了CDPS活性和可用于循环二生产的可重复使用性.
- 级联酶的联合固定是可行的和高效的,扩大生物催化剂应用的范围.
- 这种固定技术对利用复杂基质的酶如tRNA具有前景.
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