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红移B₁₂-依赖光受体蛋白通过可诱导生物材料的光学合来进行诱导

Hong Kiu Francis Fok¹, Z Yang², Xin Dai^3,4

¹Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology Clear Water Bay, Kowloon, Hong Kong SAR, 999077, China.

Angewandte Chemie (International ed. in English)

|September 6, 2024

概括

研究人员设计了可依赖于可巴胺 (B12) 的光受体,以响应红光,从而使生物材料和光遗传学中的新应用成为可能. 这种光谱调可以提高对深层组织中细胞对细胞粘附的控制.

科学领域:

合成生物学合成生物学
生物材料工程是生物材料的工程.
视觉遗传学视觉遗传学

背景情况:

科巴胺 (B12) 依存光受体对于控制生物材料中细胞对细胞粘附至关重要.
目前的B12光受体主要对绿色光反应,限制了它们在深层组织应用中的使用.

研究的目的:

开发一种将B12依赖光受体的光反应从绿色转变为红色/远红色光的总体策略.
为了使生物材料的动态控制使用红光进行更深的组织透.

主要方法:

利用醇-马莱胺点击化学方法,用红色吸光光 SulfoCyanine5 (Cy5) 标记含氨酸的 CarH_C 突变体.
采用基因编码的点击化学来组装红移光受体到水凝中.
改造了Saccharomyces cerevisiae细胞,以显示CarH_C变体,用于现场标记和红光反应.

主要成果:

被标记为CarH_C的光受体保留了依赖于adenosylcobalamin (AdoB12) 的四聚化,并获得了对红光的敏感性,导致红光暴露时的四聚体分解.
红色转移的光受体组合的水凝在对红光的反应中迅速降解.
工程酵母细胞形成了与AdoB12组装并与红光分解的生物材料.

结论:

通过光学合来调整CarH_C的光谱,提供了一种多功能方法,可以动态控制工程生物材料中细胞间的相互作用.
这种方法扩大了B12依赖光受体在光遗传学和生物材料中的实用性,特别是在深层组织应用中.

关键词:

科巴拉胺是什么意思材料科学材料科学一个光感受器.蛋白质工程工程蛋白质工程蛋白质的修改蛋白质的修改

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