在植入前胚胎发育过程中,对来自小鼠卵管的细胞外囊泡进行蛋白质组分析和体内可视化
Kalli K Stephens1, Ryan M Finnerty2,3, DeAna G Grant4
1Division of Animal Sciences, College of Agriculture, Food and Natural Resources, University of Missouri, Columbia, Missouri, USA.
概括
卵巢外细胞囊泡 (oEVs) 含有对早期胚胎发育至关重要的蛋白质. 在oEV中识别这些胚胎保护蛋白为改善体外胚胎培养提供了洞察力.
科学领域:
- 生殖生物学 生殖生物学
- 细胞外囊泡 细胞外囊泡
- 胚胎学 胚胎学
背景情况:
- 植入前的胚胎发育发生在卵管中.
- 卵巢外细胞囊泡 (oEVs) 对于胚胎发育至关重要,它含有像蛋白质一样的分子传递器.
- 了解oEV货物可以揭示当前体外胚胎培养中缺少的因素.
研究的目的:
- 在怀孕早期分离和分析OEV中的蛋白质.
- 为了比较怀孕和非怀孕状态之间的oEV蛋白质含量,以及自然与超排卵之间的比较.
- 在oEV中识别特定的胚胎保护蛋白.
主要方法:
- 在不同阶段将oEV从卵管液中分离出来.
- 对oEV的蛋白质组分析以确定蛋白质载荷.
- 传输电子显微镜和实时成像以可视化EV-胚胎相互作用.
主要成果:
- 在2306-3066个已识别的蛋白质中确定了58-60个常见的EV标记物.
- 怀孕和非怀孕样本之间的oEV蛋白质含量显著差异.
- 在胚胎的存在下丰富胚胎保护蛋白质 (例如,HMGB1,serpin).
- 与自然排卵相比,超排卵改变了oEV蛋白质组成.
结论:
- 卵巢外细胞囊中含有关键蛋白质,这些蛋白质根据妊娠状态和排卵方法不同.
- 在oEV中确定了特定的胚胎保护蛋白.
- 这些已识别的oEV蛋白具有改善胚胎发育结果的潜力,在体内和体外.
关键词:
在CD9-TIGER小鼠中.这就是TEMEM.细胞外囊泡细胞外囊泡这里是鼠标鼠标鼠标鼠标鼠标鼠标.卵管是指卵管中的卵管.卵管多索体 卵管多索体 卵管多索体怀孕 怀孕 怀孕 怀孕 怀孕在植入前的胚胎胚胎.超级排卵是一种超级排卵.更多相关视频
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