与HCV相关的Child-Pugh B级肝硬化患者的血液微生物组发生变化
Oscar Brochado-Kith1, Marta Rava2, Juan Berenguer3
1Unidad de Infección Viral e Inmunidad, Centro Nacional de Microbiología (CNM), Instituto de Salud Carlos III (ISCIII), Majadahonda, Madrid, Spain; Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, Madrid. Spain.
Journal of infection and public health
|September 6, 2024
概括
晚期肝硬化和肝功能恶化的患者 (CTP B类) 显示血液微生物多样性显著降低. 蛋白质细菌的丰富性增加与这些患者的特定代谢物有关.
科学领域:
- 微生物学 微生物学
- 肝病学 肝病学是一种肝病学.
- 代谢学 代谢学 代谢学
背景情况:
- 改变的细菌转位会影响肝功能和肝硬化进展.
- 儿童-图尔科特-普格 (CTP) 评分评估肝脏疾病的严重程度.
- 这项研究调查了基于CTP类的HCV肝硬化患者的血液微生物组和代谢组差异.
研究的目的:
- 为了比较HCV感染患者的血液微生物组和代谢组概况,CTP类B (显著妥协) 和CTP类A (补偿良好).
- 为了识别与肝功能状态相关的微生物和代谢变化.
主要方法:
- 对88名先进的HCV相关肝硬化患者进行横截面研究,分为CTP-B和CTP-A组.
- 使用MiSeq Illumina技术进行细菌16SrRNA测序.
- 通过GC-MS和LC-MS (ESI+和ESI-) 进行非向代谢.
主要成果:
- 与CTP-A.相比,CTP-B患者的微生物丰富度 (Chao1) 和α多样性 (香农,辛普森指数) 降低.
- 在族群,类和顺序层面观察到β多样性的显著差异,CTP-B的多样性较低.
- 较高的蛋白质细菌,Alphaproteobacteria,Sphingomonadales和Sphingomonadaceae的相对丰度与CTP-B有关,并与特定的代谢物如乙醇胺,油酸和p-cresol相关.
结论:
- 在CTP-B患者中,血液微生物多样性显著下降.
- 与CTP-A患者相比,CTP-B患者表现出蛋白质细菌,α蛋白质细菌,sphingomonadales和sphingomonadaceae的丰富.
- 这些微生物变化与不同的代谢概况有关,突出显示了它们在肝脏脱补偿中的作用.
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