操作条件对酸盐-DAMO系统中N2O积累的影响:动力学和微生物学分析
Yiru Zheng1, Fan Xu1, Jianwen Gan2
1School of Environmental Science and Engineering, Zhejiang Gongshang University, No. 149, Jiaogong Road, Hangzhou, 310012, China.
Journal of environmental management
|September 6, 2024
概括
依赖酸盐的厌氧甲氧化 (酸盐-DAMO) 可以产生氧化 (N2O). 这项研究优化了条件以尽量减少N2O,并揭示了pH是关键,性条件通过特定的基因增加N2O排放.
科学领域:
- 环境微生物学 环境微生物学
- 生物地质化学循环的过程
- 废水处理 废水处理
背景情况:
- 酸盐依赖性厌氧甲氧化 (Nitrate-DAMO) 是一种可持续的去除和甲的过程.
- 在酸盐-DAMO系统中,氧化 (N2O) 生产和消费的作用在很大程度上被忽视了.
- 了解N2O动态对于优化DAMO流程和减轻温室气体排放至关重要.
研究的目的:
- 调查操作参数 (C/N比,pH,温度) 对酸盐-DAMO系统中N2O积累的影响.
- 确定在酸盐-DAMO过程中最大限度地减少N2O生产的最佳条件.
- 为了阐明N2O生产和消费在酸盐-DAMO过程中的机制.
主要方法:
- 在不同操作参数下对酸盐-DAMO系统进行实验调查.
- 应用酶动力学和热力学模型来分析N2O动态.
- 多重线性回归和元基因组学测序,以确定影响N2O排放的关键因素和微生物途径.
主要成果:
- 为了最大限度地减少N2O的最佳条件被确定为C/N比率=3,pH=6.5,温度=20°C.
- 确定pH值是影响N2O积累的最重要因素.
- 性pH与N2O排放正相关,通过促进特定的脱细菌和Nor基因,同时通过Mdo基因在N2O驱动的AOM中增强N2O消耗.
结论:
- 这项研究揭示了N2O在酸盐-DAMO系统中的双重作用,即产生和消耗.
- 性pH显著影响N2O动态,通过影响微生物群落和基因表达.
- 这些发现为在实际的酸盐-DAMO应用中减少N2O排放提供了机制的理解.
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