基于青素结合蛋白的免疫试验,用于选择性和定量确定β-乳糖抗生素
Rilong Liu1, Hangzhen Lan1, Song Yan1
1State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition and College of Food Science and Engineering, Ningbo University, Ningbo 315800, China.
Enzyme and microbial technology
|September 6, 2024
概括
使用青素结合蛋白 (PBPs) 的新免疫测试方法准确量化了十种β-乳酸抗生素 (BLAs). 这种敏感的方法可以检测牛奶中的BLA含量低于欧盟限制水平,提供可靠的抗生素残留分析.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 食品安全 食品安全
背景情况:
- 贝塔乳酸抗生素 (BLA) 在畜牧业中被广泛使用.
- 确保食品安全需要敏感的方法来检测BLA残留物.
研究的目的:
- 开发和验证一种新的免疫试验,用于量化十种类型的BLAs.
- 评估使用青素结合蛋白 (PBPs) 作为检测剂的可行性.
主要方法:
- 两个关键PBP (PBP1a和PBP2x) 的表达和净化.
- 使用SDS-PAGE和西式涂抹对PBP的表征.
- 对最佳PBP-BLA相互作用的缓冲条件 (pH,离子度,有机溶剂) 的评估.
- 基于PBP的免疫试验对BLA检测的验证.
主要成果:
- 在优化条件下,PBPs表现出对BLAs的高结合亲和力.
- 免疫试验实现了高灵敏度,检测极限范围从0.21到9.12 ng/mL.
- 检测限值明显低于欧盟的最大残留限值 (4-100 ng/mL).
- 在分析牛奶样本时,获得了令人满意的恢复率 (68.9-101.3%).
结论:
- 基于PBP的免疫测试是一种敏感且有效的方法,用于定量多个BLAs.
- 开发的方法符合BLA残留物监测的严格食品安全要求.
- 这种测试为检测牛奶等食品中的抗生素残留物提供了可靠的工具.
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