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Updated: Jun 14, 2025

Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion
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从实验和计算方法的RNA动态

Giovanni Bussi1, Massimiliano Bonomi2, Paraskevi Gkeka3

  • 1Scuola Internazionale Superiore di Studi Avanzati (SISSA), Via Bonomea 265, 34136 Trieste, Italy.

Structure (London, England : 1993)
|September 6, 2024
PubMed
概括

了解RNA动态是其生物功能和治疗潜力的关键. 本报告总结了使用实验和计算方法研究RNA动态的研讨会的见解.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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