低碳和过度生产的cordycepin从甲醇使用工程Pichia pastoris细胞工厂的工程Pichia pastoris细胞工厂
Bingjie Zhao1, Yu Li1, Yong Zhang1
1College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China; Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing 100193, China.
Bioresource technology
|September 6, 2024
概括
工程酵母可以产生高产量的cordycepin,这是一个有价值的核酸类型,对环境的影响大大降低. 这一突破为使用甲醇生产cordycepin和类似化合物提供了一个可持续的替代方案.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
背景情况:
- 核糖类相似物cordycepin在医药和健康产品中至关重要.
- 目前使用Cordyceps militaris的生产方法产量低,温室气体排放量高.
- 开发高效和可持续的皮带生产是至关重要的.
研究的目的:
- 通过使用Pichia pastoris. 设计一种高产量的皮素生产菌株.
- 优化生物合成途径并增强前体供应,以增加胆素的生产.
- 与传统方法相比,评估工程菌株对环境的影响.
主要方法:
- 通过促进体组合,Kozak序列和酶融合,优化了cordycepin生物合成途径.
- 增强过氧体中的甲醇同化能力.
- 调整了NADPH和ATP的合成,加上增加了腺和3'-AMP的供应.
- 构建了高产品种Pp29并进行了摇瓶和料批发发酵.
主要成果:
- 在摇瓶发酵中构建了高产的cordycepin菌株Pp29,达到1551.44 mg/L.
- 在料批发发酵 (10L发酵器) 中,Pp29达到8.11g/L的创纪录产量.
- 与Cordyceps militaris和其他酵母系统相比,实现了显著较低的CO2-eq排放 (低于1.9-17.3倍).
结论:
- 皮奇亚牧羊树作为一种有效的底盘,用于合成使用甲醇的科迪塞宾和其他核酸类型.
- 这种工程菌株提供了一个可持续的,高收益的替代品,用于生产cordycepin.
- 这项研究为开发生物基生产有价值的核糖类相似物提供了基础.
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