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从呼吸中产生的挥发性有机化合物 (VOC) 的高质量识别
Wisenave Arulvasan1, Hsuan Chou2, Julia Greenwood2
1Owlstone Medical Ltd, Cambridge, UK. wisenave.arulvasan@owlstone.co.uk.
Metabolomics : Official journal of the Metabolomic Society
|September 6, 2024
概括
一种新方法可靠地识别出口气中的148种挥发性有机化合物 (VOC),为非侵入性诊断和标准化生物标志物发现铺平了道路.
科学领域:
- 分析化学 分析化学
- 生物标志物发现发现
- 非侵入性诊断 非侵入性诊断
背景情况:
- 呼出的气息中的挥发性有机化合物 (VOC) 为非侵入性诊断提供了潜力.
- 精确检测呼吸中的VOCs需要强大的平台来区分它们与背景污染物.
- 标准化方法对于可靠的基于呼吸的生物标志物发现至关重要.
研究的目的:
- 开发一种可靠的呼吸采集和分析方法.
- 为了生成人类呼吸中已知的VOC的全面清单.
- 建立在异质群体中发现生物标志物的基础.
主要方法:
- 使用Owlstone Medical的呼吸活检OMNI平台进行样本采集和TD-GC-MS分析.
- 从异质人群中收集了90对呼吸和背景样本.
- 使用预先定义的指标识别"呼吸"的VOC,并通过化学标准,保留索引和质谱匹配确认身份.
主要成果:
- 在所有样本的80%以上检测到1471个VOC.
- 585个挥发性有机物被确定为"呼吸"至少1度.
- 在各种化学类别中确定了148种呼吸上的VOC.
结论:
- 成功开发了一种强大的气息采集方法和VOCs的相对定量分析.
- 这项研究产生了148个真正的呼吸传播的VOCs的确认清单.
- 这份经过验证的清单将有助于未来的生物标志物发现,临床验证和跨研究标准化.
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