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Updated: Jun 14, 2025

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破坏稳定的近红外光纳米体使得基于GFP的生物传感器能够在无背景情况下进行定位,用于成像和操纵

Natalia V Barykina1, Erin M Carey2, Olena S Oliinyk3

  • 1Department of Genetics, and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York, NY, 10461, USA.

Nature communications
|September 6, 2024
PubMed
概括

研究人员开发了新的近红外光纳米体,用于基于GFP的探测器和生物传感器的无背景成像. 这些探头能够精确控制小鼠的基因表达和深层大脑体内成像.

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Protein Dynamics in Living Cells01:19

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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