相关实验视频

Updated: Jun 14, 2025

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis

Published on: June 13, 2021

在Candida albicans Cdr1的冷-EM结构显示了醇基质识别和抑制剂阻断机制

Ying Peng¹, Yan Lu¹, Hui Sun¹

¹Affiliated Hospital of Hunan University/Xiangtan Central Hospital, School of Biomedical Sciences, Hunan University, Changsha, China.

Nature communications

|September 6, 2024

概括

这项研究揭示了Candida albicans如何生长.

科学领域:

生物化学生化学
结构生物学结构生物学
菌类学菌类学是指菌类学.

背景情况:

香菌通过Cdr1排泄发展出对醇药物的耐药性.
抗寄生虫药物米尔贝米氧胺抑制Cdr1,但其机制尚不清楚.

研究的目的:

阐明Cdr1基质识别,亚醇药物输出和密尔贝米氧胺抑制的结构机制.

主要方法:

三维冷电子显微镜 (cryo-EM) 用于确定Cdr1的结构在apo,fluconazole-bound和milbemycin oxime抑制的状态.

主要成果:

冷-EM结构显示,在Cdr1的中央腔中通过疏水相互作用,可纳和密尔贝米氧化物结合.
亚醇的出口是通过一个侧向通道,涉及TM2,TM5,TM8和TM11的跨膜螺旋促进的.
米尔贝米氧胺通过与基质竞争,阻断出口和阻碍基质进入来抑制Cdr1.

结论:

这些发现澄清了Cdr1的药物识别和出口机制以及milbemycin oxime的抑制作用.
这项研究为开发新型抗真菌剂的结构性基础,以克服C. albicans的醇耐药性.

更多相关视频

Candida albicans Biofilm Chip CaBChip for High-throughput Antifungal Drug Screening

Candida albicans Biofilm Chip CaBChip for High-throughput Antifungal Drug Screening

Published on: July 18, 2012

A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms

A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms

Published on: September 16, 2022

相关概念视频

Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...