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相关概念视频

Lysogenic Cycle of Bacteriophages00:43

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In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
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Lytic Cycle of Bacteriophages01:30

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Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
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Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics
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使用细菌种群动态来计算菌体及其 lysogens.

Yuncong Geng1,2, Thu Vu Phuc Nguyen1,3,4, Ehsan Homaee1,2

  • 1Department of Physics, University of Illinois Urbana-Champaign, Urbana, IL, 61801, USA.

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概括

这项研究引入了一种高通量微板阅读器测试,以量化菌体及其 lysogens. 该方法使用光学密度来测量菌体度和宿主 lysogenization 速率,提供高灵敏度和效率.

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科学领域:

  • 微生物学 微生物学
  • 病毒学 病毒学
  • 生物物理学的生物物理.

背景情况:

  • 传统的菌体和素计数方法是低效和破坏性的.
  • 需要高通量,敏感和非扰乱的方法来量化病毒种群和宿主-病原体相互作用.

研究的目的:

  • 开发和验证基于微板阅读器的高吞吐量菌体量化试验.
  • 为了使宿主 lysogenization 率的测量.
  • 研究宿主生理学对病毒发育程序的影响.

主要方法:

  • 使用微板阅读器测量菌体感染细菌培养物的光密度 (OD) 动态.
  • 在广泛的动态范围中将溶解时的OD与初始菌体度相关联.
  • 使用数学建模推断菌体生长参数 (生长率,潜伏期,爆发大小).
  • 纳入抗生素选择以量化宿主溶解率.

主要成果:

  • 培养物溶解的OD与初始菌体度的对数呈现线性关系,使得在九个数量级上进行量化.
  • 该试验表明单菌素敏感性.
  • 菌体生长率被成功推断出来,并被证明取决于遭遇率,潜伏期和爆发大小.
  • 抗生素选择允许测量宿主溶解率.

结论:

  • 开发的微板测定是一种敏感的,高通量方法,用于量化菌体及其 lysogens.
  • 主体生理学,特别是生长速度,会影响细菌菌体的光生长和 lysogeny 倾向,如慢生长的 E. coli 中的光生长减少和 lysogeny 增加所示.
  • 这种方法为研究菌体与宿主相互作用和病毒动态提供了强大的工具.