使用细菌种群动态来计算菌体及其 lysogens
Yuncong Geng1,2, Thu Vu Phuc Nguyen1,3,4, Ehsan Homaee1,2
1Department of Physics, University of Illinois Urbana-Champaign, Urbana, IL, 61801, USA.
Nature communications
|September 6, 2024
概括
这项研究引入了一种高通量微板阅读器测试,以量化菌体及其 lysogens. 该方法使用光学密度来测量菌体度和宿主 lysogenization 速率,提供高灵敏度和效率.
科学领域:
- 微生物学 微生物学
- 病毒学 病毒学
- 生物物理学的生物物理.
背景情况:
- 传统的菌体和素计数方法是低效和破坏性的.
- 需要高通量,敏感和非扰乱的方法来量化病毒种群和宿主-病原体相互作用.
研究的目的:
- 开发和验证基于微板阅读器的高吞吐量菌体量化试验.
- 为了使宿主 lysogenization 率的测量.
- 研究宿主生理学对病毒发育程序的影响.
主要方法:
- 使用微板阅读器测量菌体感染细菌培养物的光密度 (OD) 动态.
- 在广泛的动态范围中将溶解时的OD与初始菌体度相关联.
- 使用数学建模推断菌体生长参数 (生长率,潜伏期,爆发大小).
- 纳入抗生素选择以量化宿主溶解率.
主要成果:
- 培养物溶解的OD与初始菌体度的对数呈现线性关系,使得在九个数量级上进行量化.
- 该试验表明单菌素敏感性.
- 菌体生长率被成功推断出来,并被证明取决于遭遇率,潜伏期和爆发大小.
- 抗生素选择允许测量宿主溶解率.
结论:
- 开发的微板测定是一种敏感的,高通量方法,用于量化菌体及其 lysogens.
- 主体生理学,特别是生长速度,会影响细菌菌体的光生长和 lysogeny 倾向,如慢生长的 E. coli 中的光生长减少和 lysogeny 增加所示.
- 这种方法为研究菌体与宿主相互作用和病毒动态提供了强大的工具.
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