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长跨基因非编码RNAs调节近端蛋白编码基因表达和耐受Candidatus Liberibacter spp. 的耐受性. 在土豆中
Renesh Bedre1, Naga Rajitha Kavuri1,2, Manikandan Ramasamy1
1Texas A&M AgriLife Research & Extension Center, Texas A&M University System, Weslaco, TX, USA.
Communications biology
|September 6, 2024
概括
这项研究确定了土豆植物对斑马病反应的新型长非编码RNA. 过度表达这些lincRNAs通过改变蛋白质编码基因表达来增强疾病耐受性.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因组学就是基因组学.
- 生物化学 生物化学
背景情况:
- 长跨基因非编码RNAs (lincRNAs) 调节植物中的基因表达.
- 马易患血管细菌疾病,如斑马 (ZC).
研究的目的:
- 在被Candidatus Liberibacter solanacearum (CLso) 感染的土豆植物中对全基因组的lincRNA进行鉴定.
- 调查lincRNAs在土豆对CLso感染的反应中的功能性作用.
主要方法:
- 在健康的和CLso感染的土豆植物中对lincRNAs的全基因组识别和表征.
- 分析lincRNA表达模式,接近蛋白质编码基因 (PCG) 和感染时的差异表达.
- 权重基因同表达网络分析 (WGCNA) 以确定相关的lincRNA-PCG对.
- 通过转基因土豆毛发根中选择的lincRNAs的过度表达的功能验证.
主要成果:
- 检测到大约4397个lincRNAs,其中65%是新发现的,在其他物种中很少有正义基因,这表明土豆的特异性扩张.
- 30%的lincRNAs在CLso感染后被差异表达,感染后21天出现显著变化 (DAI).
- 通过WGCNA确定了46个高度相关的lincRNA-PCG对.
- 过度表达特定的lincRNAs改变了邻近的PCG表达,并赋予了CLso耐受性.
结论:
- 马拥有涉及应激反应的多样化和大部分新的lincRNAs.
- lincRNAs在土豆对CLso的防御中发挥着功能性作用,影响PCG表达并赋予耐受性.
- 这项研究为lincRNAs在植物病原体相互作用中的调节作用提供了关键的见解.
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