重组马雷克病病毒1型对的H9N2流感A病毒提供了充分的保护
Yuntong Chen1, Qingqing Yu1, Wenrui Fan1
1State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, PR China.
Veterinary microbiology
|September 7, 2024
概括
一种使用Marek的新双价疫苗.
科学领域:
- 兽医病毒学 兽医病毒学
- 疫苗开发 疫苗开发
- 禽类的健康 禽类的健康
背景情况:
- 禽流感病毒 (AIV) H9N2亚型对家禽和公共健康构成重大威胁.
- 马雷克病病毒 (MDV) 是复合疫苗的有效载体.
- 对家禽来说,对AIV和MDV的双重保护是可取的.
研究的目的:
- 构建和评估携带H9N2 AIV血素 (HA) 基因的复合MDV1型载体.
- 评估HA基因插入部位 (UL41与US2) 对疫苗疗效的影响.
- 开发一种两价疫苗,提供对AIV和MDV的保护.
主要方法:
- 构建两个重组的MDV型1菌株:rMDV-UL41/HA和rMDV-US2/HA,其中H9N2 HA基因分别插入UL41和US2位点.
- 间接免疫光测定以确认HA蛋白表达.
- 在细胞培养中对MDV复制的评估.
- 针对特定的无病原体的免疫接种,然后对H9N2 AIV和MDV进行挑战.
主要成果:
- 这两种重组病毒都显示了持续的HA蛋白表达,并没有影响MDV复制.
- 用rMDV-UL41/HA和rMDV-US2/HA免疫接种的产生了类似的血液凝结抑制抗体标位.
- 只有rMDV-UL41/HA疫苗提供了对H9N2 AIV和MDV的完全保护.
- 在rMDV-US2/HA疫苗没有赋予完整的保护对H9N2 AIV挑战.
结论:
- 在MDV载体中H9N2 HA基因的插入部位显著影响疫苗的疗效.
- rMDV-UL41/HA菌株是针对H9N2禽流感和的马雷克病的双价疫苗的有希望的候选人.
- 针对性基因插入对于开发有效的重组病毒疫苗至关重要.
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