电穿孔后跨膜电压的长期变化是由非选择性泄漏电流和离子通道激活之间的相互作用决定的
Anja Blažič1, Manon Guinard1, Tomaž Leskovar1
1University of Ljubljana, Faculty of Electrical Engineering, SI-1000 Ljubljana, Slovenia.
Bioelectrochemistry (Amsterdam, Netherlands)
|September 7, 2024
概括
电穿孔改变了细胞膜电压,离子通道在恢复过程中发挥了关键作用. 了解这些机制,特别是激活通道,可以影响电解后的细胞反应.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 电子生理学 电子生理学
背景情况:
- 电穿孔暂时增加了细胞膜的透性.
- 电穿孔在各种细胞类型中诱导了长时间的跨膜电压变化 (TMV).
- 这些TMV变化背后的精确机制尚未完全理解.
研究的目的:
- 在不同细胞系中,在电穿孔后研究TMV变化的机制.
- 阐明离子通道在细胞对电穿孔反应中的作用.
- 探索调节电穿孔结果的潜在治疗点.
主要方法:
- 在CHO-K1和U-87 MG细胞中使用FLIPR膜电位染料对TMV进行30分钟的电解后监测.
- 使用离子通道抑制剂来探测特定通道的作用.
- 开发一个理论模型来解释观察到的TMV变化.
主要成果:
- 由于非选择性的泄漏电流,CHO-K1细胞 (低离子通道表达) 显示出脱极化,在膜重新密封时恢复静止的TMV.
- 在33°C初始脱极化后,U-87 MG细胞 (高离子通道表达) 呈现出意想不到的超极化,但在25°C时却没有.
- 一个理论模型和抑制剂实验表明,激活通道在很大程度上负责超极化.
结论:
- 离子通道活动显著影响电穿孔后的TMV动态.
- 激活通道在U-87 MG细胞中观察到的超极化反应中起着至关重要的作用.
- 向离子通道可以提供一种策略,以调节电穿孔后的细胞生物反应.
相关概念视频
The Resting Membrane Potential
131.5K
Overview
131.5K
Electrochemical Gradient and Channel Proteins: An Overview
2.1K
An electrochemical gradient is a fundamental concept in biology and chemistry. It regulates the movement of ions across cell membranes. This movement is influenced by two factors:
The electrical gradient: The electrical gradient across cell membranes refers to the difference in electric charge between the inside and outside of a cell. This difference drives the movement of ions towards or away from the cells. For instance, if the inside of the cell is more negatively charged relative to...
The electrical gradient: The electrical gradient across cell membranes refers to the difference in electric charge between the inside and outside of a cell. This difference drives the movement of ions towards or away from the cells. For instance, if the inside of the cell is more negatively charged relative to...
2.1K
Resting Membrane Potential
18.3K
The relative difference in electrical charge, or voltage, between the inside and the outside of a cell membrane, is called the membrane potential. It is generated by differences in permeability of the membrane to various ions and the concentrations of these ions across the membrane.
The Inside of a Neuron is More Negative
The membrane potential of a cell can be measured by inserting a microelectrode into a cell and comparing the charge to a reference electrode in the extracellular fluid. The...
The Inside of a Neuron is More Negative
The membrane potential of a cell can be measured by inserting a microelectrode into a cell and comparing the charge to a reference electrode in the extracellular fluid. The...
18.3K
Action Potentials
130.3K
Overview
130.3K
Action Potential
7.9K
Neurons communicate by firing action potentials—the electrochemical signal that is propagated along the axon. The signal results in the release of neurotransmitters at axon terminals, thereby transmitting information to the nervous system. An action potential is a specific "all-or-none" change in membrane potential that results in a rapid spike in voltage.
Membrane potential in neurons
Neurons typically have a resting membrane potential of about -70 millivolts (mV). When they...
Membrane potential in neurons
Neurons typically have a resting membrane potential of about -70 millivolts (mV). When they...
7.9K
Action Potential: Phases of Stimulation
5.2K
The action potential is a complex electrical event that occurs in excitable cells, such as neurons and muscle cells. It consists of several distinct phases, each with specific characteristics.
Resting Phase:
In this phase, the cell's membrane is at its resting potential, typically around -70 millivolts (mV) for neurons. Inside the cell, there is a higher concentration of potassium ions (K+) and a lower concentration of sodium ions (Na+). Voltage-gated sodium channels are closed, and...
Resting Phase:
In this phase, the cell's membrane is at its resting potential, typically around -70 millivolts (mV) for neurons. Inside the cell, there is a higher concentration of potassium ions (K+) and a lower concentration of sodium ions (Na+). Voltage-gated sodium channels are closed, and...
5.2K


