相关实验视频

Updated: Jun 14, 2025

Forming Micro-and Nano-Plastics from Agricultural Plastic Films for Employment in Fundamental Research Studies

Forming Micro-and Nano-Plastics from Agricultural Plastic Films for Employment in Fundamental Research Studies

Published on: July 27, 2022

聚烯微型和纳米塑料对雄激素和雌激素受体活性和体外类固醇生成的影响

Jeske van Boxel¹, Rani R J Khargi¹, Sandra M Nijmeijer¹

¹Amsterdam Institute for Life and Environment, section Environmental Health and Toxicology, Faculty of Science, Vrije Universiteit Amsterdam, the Netherlands.

Toxicology in vitro : an international journal published in association with BIBRA

|September 7, 2024

概括

这项研究发现微型和纳米塑料 (MNP) 对激素受体和体外类固醇生成的有限内分泌干扰作用. 需要对雌醇进行进一步的研究.

科学领域:

环境健康科学环境健康科学
毒理学毒理学毒理学
内分泌学在内分泌学.

背景情况:

塑料添加剂是已知的内分泌干扰剂,但尽管存在人类,微型和纳米塑料 (MNP) 的影响仍未得到充分研究.
由于MNP无处不在,因此需要对其潜在的内分泌干扰性质进行调查.

研究的目的:

评估各种聚钢 (PS) -MNP大小和度对雌激素和雄激素受体 (ER和AR) 活性的体外影响.
评估PS-MNP对类固醇生成和激素产生的影响.

主要方法:

暴露VM7,H295R和AR-ecoscreen细胞的光PS-MNPs (5010,000nm) 在0.01100μg/mL的度.
量化MNP内部化,ER/AR活性,类固醇激素水平和氧化应激标记基因表达 (GPX1,SOD1).

主要成果:

所有细胞类型都内化了PS-MNP,其中H295R细胞的吸收率最高.
在测试的大小和度中,没有观察到PS-MNP对ER或AR活性的显著影响.
PS-MNP诱导了荷尔蒙水平的轻微,非剂量依赖的变化,醇 (E3) 降低了高达37.5%,并增加了氧化应激标志物.

结论:

聚烯微型和纳米塑料在体外表现出有限的内分泌干扰特性.
对胎盘功能至关重要的雌醇的观察到的下降值得在怀孕期间进一步调查.

关键词:

雄激素受体的受体雄激素受体内分泌干扰干扰内分泌系统雌激素受体的受体是什么微塑料和纳米塑料的使用聚乙烯是一种聚乙烯.类固醇的产生.

更多相关视频

Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation

Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation

Published on: October 31, 2019

Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants

Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants

Published on: March 6, 2018

相关概念视频

Transducer Mechanism: Nuclear Receptors

Transducer Mechanism: Nuclear Receptors

Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:

Target Cell Response to Hormones

Target Cell Response to Hormones

Hormones intricately bind to receptors on the surface or within target cells, initiating a cascade of cellular responses.
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...

Internal Receptors

Internal Receptors

Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.