抗氧化剂通过调节活性氧物种来缓解微藻的低温压力,以改善脂质生产和抗氧化剂防御
Xueting Song1, Fanying Kong2, Bing-Feng Liu1
1State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
Bioresource technology
|September 7, 2024
概括
添加抗氧化剂,如黑激素和糖贝他因增强微藻生长和脂质生产在寒冷压力下. 这些化合物增强细胞防御,改善微藻的弹性和在较冷的气候下脂质的积累.
科学领域:
- 生物技术是生物技术.
- 微藻类生理学 微藻类生理学
- 生物化学 生物化学
背景情况:
- 微藻对于脂质生产有价值,但低温压力会抑制它们的生长和合成.
- 抗氧化剂有可能减轻压力反应,并增强微藻中的细胞功能.
研究的目的:
- 评估各种抗氧化剂对微藻生长和低温 (15°C) 脂质合成的影响.
- 评估生化指标,活性氧物种 (ROS),谷氨 (GSH) 和抗氧化酶活动的变化.
主要方法:
- 微藻在15°C下培养,其中含有不同度的抗氧化剂:丁氧化 (BHA),丁酸 (BHT),酸 (FA),氨酸 (MT),糖氨酸 (GB) 和氨酸 (Put).
- 测量了生物质度,脂质生产率,ROS水平,GSH含量和抗氧化酶活性.
主要成果:
- 最大生物质度 (1.3 g/L) 是通过100微米的黑激素 (MT) 达到的.
- 最佳的脂质生产率 (75.3 ± 5.8 mg/L d-1) 被观察到与1微米的糖贝 (GB).
- 抗氧化剂增加了GSH含量和抗氧化酶活性,增强了细胞防御低温压力.
结论:
- 特定的抗氧化剂,特别是黑和糖贝他因,在低温条件下显著改善微藻生长和脂质生产.
- 抗氧化剂的应用增强了微藻对寒冷压力的抵抗力,并为寒冷地区的脂质积累提供了基础.
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