洞察大豆蛋白分离液化物:性能特征,乳液构造和体外消化行为
Yiting Gao1, Le Chen1, Huiyue Chi1
1College of Food science, Northeast Agricultural university, Harbin, Heilongjiang 150030, China.
International journal of biological macromolecules
|September 7, 2024
概括
豆蛋白分离物 (SPI) 的酶化水解产生豆蛋白水解物 (SPHs),这些水解物与阿拉伯糖 (GA) 形成稳定的油在水 (O/W) 乳液. 这些SPH/GA乳液提供了改善的性能和有效的生物活性释放在消化过程中.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 体科学 体科学 体科学
背景情况:
- 豆蛋白分离物 (SPI) 是一种常见的食品成分,但需要修改以获得最佳的乳化.
- 酶性水解是改变蛋白质功能的一个关键技术.
- 阿拉伯 (GA) 是食品系统中广泛使用的合物乳化剂.
研究的目的:
- 研究使用大豆蛋白水解剂 (SPH) 和阿拉伯 (GA) 共同构建油在水 (O/W) 乳液.
- 评估不同酶和水解时间对SPH物理化学性质的影响.
- 将SPH/GA与SPI/GA系统的乳化性能和稳定性进行比较.
主要方法:
- 豆蛋白分离物 (SPI) 用胺,氨酸和氨酸进行过水解以产生SPHs.
- 根据酶类型和水解持续时间,分析了SPH的物理化学特性.
- 使用SPI/GA和SPHs/GA作为乳化剂制备了O/W乳液.
- 评估了乳液特性 (颗粒大小,电荷,界面蛋白质吸附,稳定性).
- 在模拟消化过程中评估了生物活性释放.
主要成果:
- 确定了最佳的水解时间:布罗美林 (BSPH2) 2小时,素 (PSPH3) 3小时,素 (TSPH3) 3小时.
- 与SPI/GA乳液相比,SPHs/GA乳液的颗粒大小更小,表面负荷更大,表面蛋白质吸附率更高.
- SPHs/GA乳液表现出增强的物理稳定性.
- 在消化过程中,从SPHs/GA乳液中观察到有效的生物活性物质释放.
结论:
- 酶性水解是修改大豆蛋白质以提高乳化能力的有效方法.
- 与SPI/GA相比,SPH/GA系统作为O/W乳液的优质乳化剂.
- SPH/GA乳液适用于需要控制释放生物活性物质的应用.
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