链杆菌 (Streptomyces avermitilis) MICNEMA2022:一种新的生物定向菌株,用于生产作为综合性线虫管理剂的亚巴麦克
Wafaa H Radwan1, Ahmed A M Abdelhafez1,2, Ahmed E Mahgoub3
1Department of Microbiology, Faculty of Agriculture, Ain Shams University, Hadayek Shoubra, P.O. Box 68, Shoubra El-Kheima, Cairo, 11566, Egypt.
BMC microbiology
|September 7, 2024
概括
一种名为MICNEMA2022的新型菌株Streptomyces avermitilis有效地产生阿巴麦克丁,这是控制根结线虫的有力药物. 这一发现为综合性线虫管理策略提供了一个生物合理的解决方案.
科学领域:
- 微生物学 微生物学
- 农业科学 农业科学
- 生物技术是生物技术.
背景情况:
- 阿巴麦丁 (ABA) 是一种来自Streptomyces avermitilis的强有力的杀虫剂和虫剂.
- 通过复杂的途径合成ABA,并使用甲醇从骨髓中提取.
- ABA被用作对抗根结线虫 Meloidogyne incognita 的生物控制剂.
研究的目的:
- 隔离和识别一种具有增强阿巴麦克丁生产能力的新菌株Streptomyces avermitilis.
- 评估隔离菌株及其阿巴麦克丁提取物对Meloidogyne incognita的杀菌效果.
主要方法:
- 选60种动态细菌分离物以确定优异的杀菌活性.
- 在体外和温室实验中评估线虫死亡率和繁殖率.
- 使用16S rRNA基因测序进行分子鉴定,并通过LC-MS和HPLC确认阿巴麦克丁的存在和数量.
主要成果:
- 单独的Streptomyces St.53表现出卓越的杀菌效果,在一天内实现了100%的体外死亡率.
- 温室试验显示,使用菌甲醇提取物显著减少线虫 (75.69%) 和低生殖率 (Rr=0.84).
- 分子鉴定证实了分离St.53作为Streptomyces avermitilis MICNEMA2022 (加入号) 的分离物. OP108264.1),LC-MS/HPLC证实了阿巴麦克B1a和B1b的生产.
结论:
- 链杆菌 (Streptomyces avermitilis MICNEMA2022) 是一种高效的阿巴麦丁生产来源.
- 已识别的菌株及其阿巴梅克丁为综合性线虫管理提供了一个有前途的生物理性剂.
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