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水生环境中的塑料碎片:一种新兴的抗微生物耐药 (AMR) 生物膜的基层

Sneha Suresh¹, S K A Ambily¹, Preethy Chandran²

¹School of Environmental Studies, Cochin University of Science and Technology, Kalamassery, Kochi, Kerala, India.

Archives of environmental contamination and toxicology

|September 8, 2024

概括

河流中的塑料垃圾可以容纳抗微生物耐药细菌,形成生物膜. 这项研究发现,聚乙烯和聚烯塑料支持耐药细菌如Bacillus物种的生物膜形成.

科学领域:

环境微生物学环境微生物学
微生物生态学微生物生态学
塑料污染研究的研究.

背景情况:

塑料污染是一个日益严重的全球问题,影响生态系统.
水生环境是塑料垃圾的储存库.
抗菌素耐药性 (AMR) 对公众健康构成重大威胁.

研究的目的:

调查塑料碎片是否作为水生环境中AMR细菌生物膜形成的基质.
为了识别与Periyar河中的塑料垃圾相关的AMR细菌.
分析在塑料表面上选择的AMR细菌的生物膜形成潜力.

主要方法:

从印度佩里亚尔河的六个地点收集塑料垃圾.
福里埃变换红外光谱 (FTIR) 用于塑料识别 (聚乙烯,聚烯).
抗生素耐药性查和多重抗生素耐药性 (MAR) 指数的计算.
16sRNA测序用于细菌识别.
在不同介质中的塑料样本上使用选定的细菌菌株进行体外生物膜形成试验.
扫描电子显微镜 (SEM) 用于表面形态分析.

主要成果:

发现塑料碎片 (聚乙烯和聚烯) 含有AMR细菌.
所有细菌分离物表现出100%的抗素耐药性,MAR指数在0.1-0.4.4之间.
鉴定了六种细菌物种,包括菌菌和菌大麦.
在不同的介质中观察到显著的生物膜生长 (P < 0.05),独立于塑料类型 (P > 0.05).
在SEM分析中,发现了细胞外聚合物质 (EPS) 和表面变化,这些变化表明了生物膜的形成.

结论:

水生环境中的塑料碎片作为AMR细菌生物膜形成的可行基质.
这些发现强调了塑料污染在耐药细菌的增殖和生存中的作用.
需要进一步的研究来了解对水质和公共卫生的影响.

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