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龙徐通古囊通过调节氧化应激和线粒体功能障碍来缓解缺血/再输诱导的心肌细胞亡
Pei-Xun Yang1, Xiao-Xue Fan2, Min-Xuan Liu3
1State Key Laboratory on Technologies for Chinese Medicine Pharmaceutical Process Control and Intelligent Manufacture, Jiangning Industrial City, Economic and Technological Development Zone, Lianyungang, Jiangsu 222001, China; Kanion School of Chinese Materia Medica, Nanjing University of Chinese Medicine, 138 Xianlin Avenue Qixia District, Nanjing 210046, PR China; Jiangsu Kanion Pharmaceutical Co Ltd, Jiangning Industrial City, Economic and Technological Development Zone, Lianyungang, Jiangsu 222001, China.
长寿口红囊 (LTC) 通过抑制氧化应激和改善线粒体功能来缓解心肌缺血-再输损伤 (MIRI). 这项研究表明LTC是MIRI治疗的潜在治疗剂.
科学领域:
- 心血管研究研究心血管研究
- 线粒体生物学 线粒体生物学
- 氧化压力研究 研究 氧化压力研究
背景情况:
- 中国龙血树脂 (Dracaena cochinchinensis) 是治疗心血管疾病的传统药物.
- 从龙血中获得的Longxuetongluo囊 (LTC) 用于缺血性中风.
- 心肌缺血-反损伤 (MIRI) 涉及复杂的机制,LTC的作用正在研究中.
研究的目的:
- 研究LTC对MIRI的心脏保护作用.
- 阐明LTC在缓解MIRI方面的潜在机制,重点关注线粒体功能障碍和氧化应激.
主要方法:
- 已建立的老鼠MIRI和H9c2心肌细胞OGD/R模型.
- 给药LTC和评估心脏病发作大小,心脏组织学和血清酶.
- 评估了亡,活性氧物种 (ROS),线粒体膜潜力 (ΔΨm) 和Ca2+水平.
- 分析了线粒体形态,动力学 (Drp1) 和相关蛋白质表达通过染色和西方涂抹.
主要成果:
- LTC治疗减少了心肌梗塞区域和心脏酶水平,改善了MIRI.
- 通过维持ΔΨm,减少ROS和Ca2+积累,调节线粒体动态,LTC抑制了心肌细胞亡并恢复了线粒体功能.
- LTC降低了NADPH氧化酶成分 (NOX2,NOX4,p67phox) 的表达,从而减少了氧化应激.
结论:
- 通过抑制ROS生成和减轻线粒体功能障碍,LTC证明了对MIRI的心脏保护作用.
- 这些发现支持LTC作为MIRI的潜在治疗策略.
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