相关实验视频

Updated: Jun 14, 2025

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

Published on: February 10, 2023

揭示了Eleutheronema tetradactylum中的度调节和免疫适应,通过高通量单细胞转录组测序

Xiaoli Ma¹, Wen-Xiong Wang¹

¹School of Energy and Environment and State Key Laboratory of Marine Pollution, City University of Hong Kong, Kowloon, Hong Kong, China; Research Centre for the Oceans and Human Health, City University of Hong Kong Shenzhen Research Institute, Shenzhen, 518057, China.

Fish & shellfish immunology

|September 8, 2024

概括

这项研究揭示了四指线鱼中的多样性细胞类型,揭示了它们在透调节和免疫力中的作用. 它提供了一个细胞地图,以了解这种重要的鱼类物种适应变化的盐度.

科学领域:

水生生物学水生生物学
鱼类生理学鱼类生理学
基因组学就是基因组学.

背景情况:

四指线鱼 (Eleutheronema tetradactylum) 在经济上很重要,可以承受不同的盐度.
是透调节和免疫的关键,但E. tetradactylum的细胞细节是未知的.
之前的研究缺乏关于状功能和适应的细胞水平洞察力.

研究的目的:

用单细胞RNA测序来表征E. tetradactylum中的细胞异质性.
识别不同细胞类型的新基因标记物和功能.
为了研究细胞对盐度波动的反应.

主要方法:

高通量单细胞RNA测序 (scRNA-seq) 的E. tetradactylum.
细胞类型和标记基因的识别和注释.
针对盐度反应的权重基因联合表达网络分析 (WGCNA).

主要成果:

在E. tetradactylum中描述了16种不同的细胞类型.
鉴定了每个细胞类型的独特基因标记物和丰富的功能.
将离子细胞细分为四个新的亚群,并揭示了盐度反应机制.

结论:

建立了一个全面的E. tetradactylum的细胞类型地图.
证明了状细胞在透调节和免疫防御中的复杂作用.
提供了关于欧鱼物种保护和管理的适应性战略的见解.

关键词:

埃莱瑟罗内马四度动态.部部部部盐度适应的适应情况这就是scRNA-seqq.

更多相关视频

A Time Differential Staining Technique Coupled with Full Bilateral Gill Denervation to Study Ionocytes in Fish

A Time Differential Staining Technique Coupled with Full Bilateral Gill Denervation to Study Ionocytes in Fish

Published on: March 19, 2015

Preparation of a High-quality Primary Cell Culture from Fish Pituitaries

Preparation of a High-quality Primary Cell Culture from Fish Pituitaries

Published on: August 28, 2018

相关概念视频

Osmoregulation in Fishes

Osmoregulation in Fishes

When cells are placed in a hypotonic (low-salt) fluid, they can swell and burst. Meanwhile, cells in a hypertonic solution—with a higher salt concentration—can shrivel and die. How do fish cells avoid these gruesome fates in hypotonic freshwater or hypertonic seawater environments?

Osmoregulation in Insects

Osmoregulation in Insects

Malpighian tubules are specialized structures found in the digestive systems of many arthropods, including most insects, that handle excretion and osmoregulation. The tubules are typically arranged in pairs and have a convoluted structure that increases their surface area.