在潜在的内心炎患者队列中,使用外周血液转录组学识别宿主内型
Israel David Duarte-Herrera1, Cecilia López-Martínez2, Raquel Rodríguez-García3
1Centro de Investigación Biomédica en Red (CIBER)-Enfermedades Respiratorias, Madrid, Spain; Instituto de Investigación Sanitaria del Principado de Asturias, Oviedo, Spain.
概括
在感染性内心炎患者中,基因表达确定了两种不同的宿主反应概况 (内型). 这些内型,EE1和EE2,表现出不同的分子机制,手术反应和明显不同的死亡率.
科学领域:
- 传染性疾病 传染性疾病
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
背景情况:
- 主体反应显著影响感染结果.
- 感染性内心炎的不同患者应答概况仍然没有被描述.
研究的目的:
- 利用转录学来识别感染性内心炎患者的不同内型.
- 描述已识别的内型之间临床数据,基因表达和结果的差异.
主要方法:
- 在诊断时对32名感染性内心炎患者的血液样本进行转录学分析.
- 聚类基因表达数据来定义两个内型:EE1和EE2.
- 解构以确定活跃细胞群并评估途径和存活率的差异.
主要成果:
- 确定了两个内型 (EE1,n=18;EE2,n=14) 具有相似的临床数据但不同的基因表达特征.
- EE2患者表现出丰富的T细胞成熟基因,更高的活性T细胞和更低的中性粒细胞.
- 与EE2患者相比,EE1患者的住院死亡率明显高 (56%vs14%).
结论:
- 基因表达分析揭示了急性传染性内心炎的两个不同的内型.
- 这些内型在病原遗传机制,对手术干预的反应和患者的结果上有所不同.
- 这些已识别的内型也在COVID-19和败血症患者中观察到,这表明更广泛的适用性.
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