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描述C17.2细胞系作为内分泌干扰诱导的发育神经毒性的测试系统

Andrea Cediel-Ulloa¹, Roseline Awoga^1,2, Arif Dönmez³

¹Department of Organismal Biology, Uppsala University, Uppsala, Sweden.

|September 9, 2024

概括

这项研究表明,C17.2神经原生细胞可以检测由内分泌干扰物引起的发育神经毒性. 这些细胞对特定的激素受体激动剂做出反应,有助于评估内分泌干扰引起的DNT的危险性.

科学领域:

神经科学是一个神经科学.
内分泌学在内分泌学.
毒理学毒理学毒理学

背景情况:

激素信号传递对于胎儿大脑发育至关重要.
内分泌干扰化学物质 (EDC) 可以干扰发育期间的荷尔蒙信号,导致神经发育问题.
需要进行测试来评估EDCs引起的发育神经毒性 (DNT).

研究的目的:

为了评估C17.2神经原始细胞系作为检测内分泌干扰诱导的DNT的体外试验系统.
评估C17.2细胞对各种激素受体激动剂和抗剂的反应能力.

主要方法:

C17.2细胞分化了10天.
细胞暴露于甲状腺激素 (THR),葡萄糖皮质 (GR),视网膜酸 (RAR),视网膜X (RXR),氧醇 (LXR),雌激素 (ER),雄激素 (AR),过氧酶增殖器激活的三角体 (PPARβ/δ) 和维生素D (VDR) 受体的激素/对抗体.
神经元形态 (神经元外生,分支) 和神经元分化百分比通过免疫光染色测定βIII-tubulin.

主要成果:

RAR,RXR和PPARβ/δ激动剂降低了神经元的外生长和分支.
暴露于GR激动剂增加了神经元分化.
暴露于RXR激动剂减少了神经元分化.

结论:

C17.2细胞表现出对GR,RAR,RXR和PPARβ/δ激动剂的反应.
C17.2细胞系显示出作为一种有价值的体外系统来评估由内分泌干扰引起的DNT危险性的潜力.

关键词:

内分泌干扰物干扰内分泌干扰物在体外检测测的发展.神经发育的神经发育过氧体增殖器激活受体的受体.网红酸是什么意思网红酸是什么意思

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