组织病理学分析有可能揭示内分泌相关瘤中的激素信号传递
Yasuhiro Miki1, Erina Iwabuchi2, Chihiro Inoue1
1Department of Anatomic Pathology, Tohoku University Graduate School of Medicine, Sendai, Japan.
Endocrine oncology (Bristol, England)
|September 9, 2024
概括
使用FFPE组织可视化人类瘤中的类固醇激素信号,有助于癌症治疗. 本综述详细介绍了分析甲固定嵌组织中的激素通路的方法,以更好地诊断和治疗.
科学领域:
- 内分泌学和瘤学 在内分泌学和瘤学.
- 分子生物学和病理学 分子生物学和病理学
背景情况:
- 类固醇激素信号传递对荷尔蒙依赖性瘤至关重要,但目前的研究方法通常仅限于体外或动物模型.
- 分析人类病理组织对于直接临床应用至关重要,但甲固定嵌 (FFPE) 组织对分子分析构成挑战.
- 传统的方法,如免疫组织化学,对于FFPE组织分析在癌症诊断和治疗中至关重要.
研究的目的:
- 审查可视化方法,以阐明在荷尔蒙依赖性瘤中的类固醇激素信号通路.
- 突出人类病理性FFPE组织样本对于理解癌症中的激素信号的有用性.
- 讨论克服FFPE组织分析局限性的技术.
主要方法:
- 免疫组织化学用于检测FFPE组织中的受体,酶和反应蛋白.
- 在现场近距离结合试验用于检查蛋白质-蛋白质相互作用 (例如受体二极体).
- 西南基因化学 分析FFPE组织中的DNA结合蛋白.
主要成果:
- 这些方法使得在与激素相关的瘤中能够对类固醇激素信号通路进行基因病理学分析.
- 尽管存在潜在的变质,但FPE组织通过显微镜提供了回顾性研究能力和空间信息.
- 可视化技术允许详细检查瘤微环境中的激素信号组件.
结论:
- 分析FFPE组织提供了明确的激素信号与激素依赖性瘤的临床治疗/诊断之间的直接联系.
- 先进的检测方法,包括免疫组织化学和现场检测,是克服FFPE组织限制的关键.
- 这种方法有助于全面地对癌症中类固醇激素信号的全身病理学理解,为改进的治疗策略铺平了道路.
相关概念视频
01:08Chemical Signaling in the Endocrine System
3.1K
A signaling cascade is a series of events that facilitates the transmission of information within or between cells, culminating in a targeted response in the recipient cell. As chemical messengers, hormones are pivotal in initiating and modulating these intricate signaling cascades based on their solubility.
Lipid-soluble hormones, such as steroid hormones, demonstrate an intracellular action. These hormones traverse cell membranes due to their lipid nature. Once inside the target cell, they...
Lipid-soluble hormones, such as steroid hormones, demonstrate an intracellular action. These hormones traverse cell membranes due to their lipid nature. Once inside the target cell, they...
3.1K
01:19Regulation of Hormone Secretion
3.3K
Regulation of hormone secretion is a finely tuned orchestration driven by various types of stimuli, encompassing neural, humoral, and hormonal signals. Environmental cues instigate neural stimuli, where action potentials traverse nerve fibers to reach their designated targets. An illustrative scenario is the body's response to stress, wherein the sympathetic nervous system releases epinephrine from the adrenal glands, inducing the well-known 'fight or flight' reaction.
Humoral...
Humoral...
3.3K
01:22Target Cell Response to Hormones
3.0K
Hormones intricately bind to receptors on the surface or within target cells, initiating a cascade of cellular responses.
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...
3.0K
00:59Structures of the Endocrine System
7.3K
The intricate framework of the endocrine system encompasses a diverse array of glands, with their target tissues and organs strategically distributed throughout the body. Central to this network are the endocrine glands, specialized structures that lack ducts and release hormones directly into the interstitial fluid. Notably, the hypothalamus, a vital neuroendocrine organ situated in the brain, governs neural functions and serves as a potent source of hormonal regulation. Near the hypothalamus...
7.3K