在Streptococcus mutans中Mutacin基因类型的频率 隔离于口腔潜在恶性疾病 (OPMD) 患者
Srudhika Sakthivel1, A S Smiline Girija1, Vijayashree J Priyadharsini1
1Department of Microbiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS) Deemed University, Chennai, IND.
在口腔潜在恶性疾病 (OPMD) 患者中分析了Streptococcus mutans流行率和突变蛋白基因表达. 在OPMD和虫群体中发现了 mutacin 基因,这表明它在口腔疾病中起着作用.
科学领域:
- 口腔微生物学 口腔微生物学
- 分子生物学分子生物学
- 牙科研究 牙科研究
背景情况:
- 转基因是Streptococcus mutans中的毒性因子,有助于口腔牙疾病.
- 口腔潜在恶性疾病 (OPMDs) 是口腔粘膜层的前恶性状况.
研究的目的:
- 从OPMD患者的临床样本中表现出Streptococcus mutans的表型特征.
- 评估与健康个体相比,来自OPMD患者的S. mutans中突变酶基因的频率.
主要方法:
- 收集了三个组的60名个体的唾液样本.
- 突变性菌 Streptococcus mutans 分离物被确定为表型.
- 使用聚合酶链反应 (PCR) 来评估mutacin基因的存在和类型.
主要成果:
- 在OPMD病例中,Streptococcus mutans的患病率高 (45%) 比患有的健康个体 (15%) 更高.
- 转基因在OPMD和虫群体中得到表达,但在没有虫的健康个体中没有表达.
- 穆塔I表达率最高 (88%在OPMD,62.5%在虫组);穆塔III表达率为0%.
结论:
- 该研究确定了与OPMD和空相关的临床Streptococcus mutans菌株中的突变酶基因类型.
- 需要进一步的研究来确定频率并探索针对这些基因的新药标.
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