基于扩散性的对比增强计算机断层扫描 (diceCT) 协议的实验评估
Lucy F Costello1, Hazel L Richards2, Alistair R Evans2,3
1Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia.
PeerJ
|September 9, 2024
概括
扩散性基对比增强计算机断层扫描 (diceCT) 能够有效地拍摄高达4.5公斤的较大标本. 最佳的方案需要不到5周的卢戈尔的治疗,以尽量减少软组织体积变化.
科学领域:
- 比较解剖学的比较解剖学.
- 放射性成像学成像学
- 数字形态学数字形态学
背景情况:
- 扩散性基对比增强型计算机断层扫描 (diceCT) 在CT成像中增强软组织对比度.
- 目前的diseCT协议主要针对1公斤以下的标本进行了优化.
- 对于较大的标本 (超过1公斤) 缺乏标准化的协议.
研究的目的:
- 实验性评估diseCT协议对重量为1-4.5公斤的全身标本的疗效.
- 为了确定Lugol的度,持续时间和样本准备对成像结果的影响.
- 量化软组织的放射密度和随时间变化的体积变化.
主要方法:
- 在8周的时间内对澳大利亚毛刺 (1-4.5公斤) 进行了连续CT成像.
- 卢戈尔的度,皮肤的存在/不存在,溶液体积和变.
- 每周对组织放射密度的监测和体积变化的量化.
主要成果:
- 显著的软组织辐射密度和体积变化发生在Lugol治疗的前28天内.
- 剥皮较大的标本为溶液扩散提供了微不足道的好处.
- 高Lugol的度 (10%) 和长期暴露 (超过5周) 导致软组织体积变化显著.
结论:
- 已建立的diseCT协议对于较大的标本 (1-4.5公斤) 是有效的.
- 优化方案应考虑治疗持续时间在5周以下,以减轻体积扭曲.
- 发现指南 diceCT协议设计,用于1公斤以上的哺乳动物标本.
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