系统的代谢工程使Saccharomyces cerevisiae中高效生产维生素A成为可能
Yi Shi1,2, Shuhuan Lu3, Xiao Zhou1,2
1Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.
Synthetic and systems biotechnology
|September 9, 2024
概括
这项研究将酵母 (*Saccharomyces cerevisiae*) 改造为产生维生素A,达到创纪录的5.21g/L. 这一突破为生物基维生素A生产提供了一种可持续和经济的方法.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 维生素A对于许多生物功能至关重要,在各个行业中广泛使用.
- 使用合成生物学的微生物生产为传统方法提供了一个可持续的替代方案.
- 麦片是一种安全和多功能模型生物体,用于代谢工程.
研究的目的:
- 为了开发一种高度维生素A生产菌株的Saccharomyces cerevisiae.
- 优化代谢途径,以有效地将β-胡卜素转化为维生素A.
- 建立一个基础,以商业规模生产生物基维生素A.
主要方法:
- 挖掘和表达β-胡卜素15,15'-单二氧化酶异酶 (Mbblh,Ssbco).
- 引入人类视网醇脱酶12以调节维生素A成分.
- 通过POS5Δ17过度表达来提高辅因子的可用性.
- 优化基因表达通过多副本整合速度限制基因.
主要成果:
- 使用摇瓶发酵实现了90%以上的网醇纯度.
- 通过增强NADPH池,增加了46%的维生素A标位.
- 在摇瓶中达到588mg/L的标位.
- 在1L生物反应器中达到5.21g/L的最高报告标位.
结论:
- 工程化Saccharomyces cerevisiae显示了可持续和经济的维生素A合成的巨大潜力.
- 开发的菌株和方法为工业规模的生物基维生素A生产铺平了道路.
- 这项研究有助于推进微生物生产必需营养素的领域.
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