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交替的随机两多硫化物:聚合,酶活性抑制和氧化还原反应性客体释放

Sukanya Bera¹, Suhrit Ghosh¹

¹School of Applied and Interdisciplinary Sciences, Indian Association for the Cultivation of Science, 2A and 2B Raja S. C. Mullick Road, Kolkata, India 700032. psusg2@iacs.res.in.

|September 9, 2024

概括

这项研究合成了一种具有生物可降解骨干的交替共聚物 (ACP),证明了其独特的自我组装成稳定的纤维状网络. 这种有序结构增强了动力稳定性,并改善了与随机共聚物相比的酶活性抑制.

科学领域:

聚合物化学聚合物化学
材料科学材料科学材料科学
生物材料是一种生物材料.

背景情况:

两性共聚合物对于自组装和药物输送至关重要.
控制单体序列会影响聚合物的特性和组装行为.
生物可降解的脊柱提供了可控降解和释放的潜力.

研究的目的:

合成和表征一种具有生物可降解聚硫化物骨干的交替共聚物 (ACP).
研究交替单体连接对自我组装,形态和运动稳定性的影响.
为了评估药物释放动力学和酶活性抑制的ACP.

主要方法:

凝聚聚合的聚合物合成两性交替共聚合物 (P1) 和随机共聚合物 (P2).
动态光散射,WXRD和冷TEM用于形态和结构分析.
光共振能量转移 (FRET) 来评估链交换动态和运动稳定性.
谷氨诱导释放研究和酶活性抑制试验.

主要成果:

ACP P1形成了超薄,纠的纤维状网络,具有晶体性质,与随机共聚物P2的圆盘状形态不同.
P1表现出显著增强的动力稳定性与最小的链交换,由FRET研究证实.
对于P1,谷氨诱导的药物释放速度较慢,与其稳定组装相关.
由于卓越的表面功能组显示和静电相互作用,P1有效抑制了α-chymotrypsin活性 (>70%).

结论:

在ACP P1中完美的交替序列驱动独特的结晶驱动的自我组装到高度稳定的纤维状网络中.
增强的P1组件的动力稳定性转化为可控的药物释放和有效的酶活性抑制.
这项研究强调了单体序列在设计功能性两性共聚合物的关键作用,用于先进的应用.

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