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Sly-miR398b通过调配auxin和H2O2 在西红中发出信号来调节成熟叶片的平坦化
Xinshan Zhang1, Xiujuan Wang1, Fei Deng2
1Discipline of Facility Horticulture, College of Horticulture Science, Zhejiang A&F University, Hangzhou, Zhejiang, China.
Plant, cell & environment
|September 9, 2024
概括
番茄微RNAsly-miR398b通过调节过氧化和auxin来维持成熟的叶子平坦化. 干扰sly-miR398b会导致叶子表皮,但奥克辛的应用会恢复平整.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 植物生理学 植物生理学
背景情况:
- 叶子平整对于光合作用至关重要,但在成熟的叶子中维持叶子平整的机制尚不清楚.
- 以前的研究集中在早期的叶子发育,留下成熟的叶子维护研究不足.
研究的目的:
- 调查sly-miR398b在保持番茄成熟叶片平坦化中的作用.
- 为了阐明涉及sly-miR398b,过氧化和auxin的分子途径.
主要方法:
- 在现场杂交以确定sly-miR398b表达式.
- 通过CRISPR/Cas9基因编辑来破坏sly-miR398b.
- 对目标基因SlCSD1表达和过氧化水平的分析.
- 激素治疗 (H2O2,NAA) 以评估对叶子形态和基因表达的影响.
主要成果:
- sly-miR398b在斜叶侧表达,其破坏导致非对称的SlCSD1表达.
- 这种不对称性增加了无轴过氧化,改变了辅酶的运输和减少了无轴辅酶的反应,导致了叶子表皮.
- 外源的H2O2诱导了表皮质,而NAA治疗则在突变分子中挽救了叶子平坦化.
结论:
- sly-miR398b对于保持番茄成熟的叶子平整至关重要.
- 这一过程中,sly-miR398b/SlCSD1/H2O2/auxin信号通路至关重要.
- 这些发现为叶子形态的荷尔蒙调节提供了新的见解.
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