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Updated: Jun 13, 2025

Author Spotlight: Quantification of Complex Lipidomic Samples Using Stable Isotope Labeling
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使用稳定同位素标记 LC-TIMS-TOF MS/MS 的 de novo 脂质的追踪

Katherine D Castro1, Lilian Valadares Tose1, Matthew Willetts2

  • 1Deparment of Chemistry and Biochemistry, Florida International University.

Journal of visualized experiments : JoVE
|September 9, 2024
PubMed
概括

稳定同位素标记 (SIL) 通过使脂质合成和代谢的高通量分析,有助于脂质研究. 这种新方法有效地识别了生物样本中的新型脂质结构,比如开发蚊子卵巢.

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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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