流量通过膜潜力调节细菌基本生理学的变化
Emily E Whittle1, Oluwatosin Orababa1, Alexander Osgerby2
1Department of Microbes, Infection and Microbiomes, Institute of Microbiology and Infection, College of Medical and Dental Sciences, Birmingham, United Kingdom.
禁用像AcrB这样的细菌排泄会延迟进入静止阶段,改变新陈代谢,并可能增加抗生素的有效性. 这会影响细菌生理学和能量生产.
科学领域:
- 微生物学 微生物学
- 细菌生理学 细菌生理学
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 排泄可以从细菌中去除抗生素,从而促进抗菌素耐药性.
- 流出功能丧失主要在活跃生长的细胞中观察到.
- 沙门氏菌 Typhimurium 的外在生长过程中发生变化,影响了抗生素的透性.
研究的目的:
- 调查删除沙门氏菌Typhimurium的主要排泄 (AcrB) 对全球基因转录的影响.
- 了解AcrB删除如何影响细菌生长,新陈代谢和膜潜力.
- 探索将排水与过渡到静止相联系起来的调节机制.
主要方法:
- 在Salmonella Typhimurium中删除了AcrB排水.
- 在不同生长阶段进行全球转录组分析.
- 对膜潜力的测量和对基因表达调节者的分析.
主要成果:
- AcrB淘汰菌株进入静止阶段的时间比野生类型晚.
- 观察到厌氧能量代谢基因的表达增加和延长.
- 删除AcrB导致膜潜力增加和超极化.
- 提出了一个模型,ArcBA感知了氧化还原状态的变化,以调节生长阶段过渡.
结论:
- 排泄的停用显著改变了细菌生理学和新陈代谢.
- 溢出在维持膜潜力和能量代谢方面发挥着至关重要的作用.
- 抑制排泄可能通过延长积累和可能增加反应性物种生成来增强抗生素活性.
- 由于排泄失活,细菌生理上的变化可能解释了突变物中的毒性降低.
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