快速生长的Vibrio natriegens的工程,用于生物合成聚3-基酸-乳酸)
Xinye Sun1, Yanzhe Shang2, Binghao Zhang1
1State Key Laboratory of Bioreactor Engineering, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
Bioresources and bioprocessing
|September 9, 2024
概括
这项研究成功地在Vibrio natriegens.中产生了聚3-基甲酸-协乳酸) [P(3HB-co-LA] . 基因改造增加了乳酸盐含量,表明V. natriegens是可生物降解聚合物生产的有前途的宿主.
科学领域:
- 生物技术是生物技术.
- 聚合物科学 聚合物科学
- 微生物工程 微生物工程
背景情况:
- 聚3-基酸-协乳酸) [P(3HB-co-LA) ]是一种具有理想生物相容性和可降解性的可生物降解材料.
- 维布里奥纳特里根是一种快速生长的细菌,具有广泛的基质范围,使其成为生物聚合物生产的合适候选者.
研究的目的:
- 为了设计Vibrio natriegens用于生产P(3HB-co-LA).
- 为了确定关键的酶和基因修饰,以优化P(3HB-co-LA) 组成.
- 用不同的碳来源来描述P(3HB-co-LA) 的生产.
主要方法:
- 鉴定乙转移酶PN96-18060在聚基酸盐 (PHB) 合成中的作用.
- 在V. natriegens.中引入异构的P(3HB-co-LA) 合成途径.
- 过度表达dldh基因以增加乳酸合并.
- 使用各种碳来源进行发酵研究,以分析P(3HB-co-LA) 生产.
主要成果:
- 在V. natriegens.中成功地产生P(3HB-co-LA) 的异质生成.
- 过度表达DLDH导致乳酸含量增加1.84倍.
- 在P(3HB-co-LA中达到28.3mol%的乳酸分数,明显高于对照组.
- 在不同的碳来源下证明了P(3HB-co-LA) 的生产.
结论:
- 振动性纳特里基因是生产P(3HB-co-LA) 的可行和高效宿主.
- 基因工程策略,包括DLDH过度表达,可以有效地控制共聚合物组成.
- 这项工作代表了在V. natriegens中首次成功生产P(3HB-co-LA),突出了其对其他共聚合物的潜力.
相关概念视频
Bioremediation
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
Upstream Processing
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
Production of Antibiotics
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...


