综合电化学Aptamer生物传感和色度pH监测通过水凝微针测试评估抗生素治疗
Fatemeh Keyvani1, Peyman GhavamiNejad1, Mahmoud Ayman Saleh2
1Department of Electrical and Computer Engineering, Faculty of Engineering, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|September 9, 2024
概括
一种新型的微针生物传感器可以实时,最少侵入性治疗药物监测万科米辛和甘他米辛. 与传统的血液检测相比,这种创新减少了处理时间,改善了抗生素治疗管理.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 目前的治疗药物监测 (TDM) 依赖于血液样本,导致实验室处理的重大延迟.
- 需要最小侵入性和实时监测方法来优化抗生素治疗和患者的结果.
研究的目的:
- 开发一个微针 (MN) 生物传感器,用于实时,最少侵入性监测万科米辛 (VAN) 和胺素 (GEN) 水平.
- 创建一个补充MN生物传感器用于pH监测,以评估抗生素治疗期间患者的反应.
主要方法:
- 一个水凝微针 (HMN) 集成与一个aptamer功能化的柔性电极 (Flex) 被设计 (HMN-Flex) 来提取间歇性液体 (ISF) 和检测抗生素.
- 为了检测pH值,开发了一种使用色度测量方法的单独HMN-pH测定.
- 使用皮肤模型,动物模型和通过多重检测来验证性能.
主要成果:
- 该HMN-Flex系统成功监测了ISF中的VAN和GEN水平,使得药理动力学分析能够与基于血液的方法相提并论.
- 该HMN-pH测定证明了有效的pH监测在体外和体内.
- 在活体动物模型中实现了同时检测抗生素和pH值.
结论:
- 开发的微针生物传感器为实时,最小侵入性抗生素TDM和生理监测提供了有希望的方法.
- 这项技术有可能通过提供即时反来显著改善抗生素治疗策略和患者护理.
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