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在宿主-病原体相互作用期间的初级代谢和相关基因表达的变化在Brassica napusus的球根耐药性

Aleya Ferdausi¹, Swati Megha¹, Nat N V Kav¹

¹Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada.

|September 9, 2024

概括

主要新陈代谢影响了Brassica napus.中的球根抗性. 耐药的焦油菜线显示出更高的酸和脂肪酸积累,而易受性线则积累了更多的有机酸.

科学领域:

植物病理学植物病理学
植物生物化学植物生物化学
分子生物学分子生物学

背景情况:

球根病是由Plasmodiophora brassicae引起的,是布拉西卡作物的毁灭性疾病.
主要新陈代谢在Brassica napus对球根的耐药性中的作用仍未得到充分研究.

研究的目的:

在Brassica napus-Plasmodiophora brassicae相互作用期间调查主要代谢物和基因表达.
确定关键的代谢途径和与球根抵抗相关的化合物.

主要方法:

用P. brassicae病变型3A.接种耐药和易受性甘露菌株的注射.
在注射后7,14,21天内源性初级代谢物的量化.
用qRT-PCR对选定的生物合成途径基因进行基因表达分析.

主要成果:

聚氨酸 (精氨酸,精氨酸) 与球根易感性有关.
在较晚的感染阶段,耐药菌株表现出更高水平的谷氨酸,甲胺和酸 (SA).
敏感线积累了较高水平的有机酸;外源性SA应用减少了敏感B. napus.的疾病严重程度.
耐药系表现出脂肪酸积累增加和GPD和AAPT基因的上调.

结论:

主要代谢物,特别是酸和脂肪酸,在Brassica napus球根耐药性方面发挥着重要作用.
特定的氨基酸和有机酸与敏感性或耐药性有关.
代谢分析提供了对宿主-病原体相互作用和潜在的耐药性机制的见解.

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