Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

750
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
750
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K
Chemical Ionization (CI) Mass Spectrometry01:21

Chemical Ionization (CI) Mass Spectrometry

710
The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
710
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

4.7K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
4.7K
Mass Spectrometers01:16

Mass Spectrometers

5.2K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.2K
Mass Spectrometry: Carboxylic Acid, Ester, and Amide Fragmentation01:01

Mass Spectrometry: Carboxylic Acid, Ester, and Amide Fragmentation

1.2K
The fragmentation patterns observed for compounds such as carboxylic acids, esters, and amides in the mass spectra include ⍺-cleavage and McLafferty rearrangement. Fragmentation by ⍺-cleavage preferentially occurs at the carbon-carbon bond at the ⍺-position next to the carboxylic group to generate a neutral radical and a cation. Long chain compounds with hydrogen at their γ-carbon undergo McLafferty rearrangement to give a radical cation and a neutral alkene.
For example,...
1.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Accurate prediction of asparagine deamidation in biologics using advanced machine learning models.

Briefings in bioinformatics·2026
Same author

Engineering nanoparticle surface chemistry for antigen-presenting cell targeting improves specificity and safety of TLR3 agonist cancer immunotherapy.

bioRxiv : the preprint server for biology·2026
Same author

Polyelectrolyte nanoparticles enable intracellular delivery of STING protein fragments for ovarian cancer immunotherapy.

Materials today. Bio·2026
Same author

A multivalent peptide-polymer conjugate material mimics STING to therapeutically activate innate immune signaling.

bioRxiv : the preprint server for biology·2026
Same author

Analysis of Nanosensor-Reported Waveforms for Plant Wounding.

Nano letters·2026
Same author

Accelerating antibody development: sequence and structure-based models for predicting developability properties via size exclusion chromatography.

mAbs·2025

相关实验视频

Updated: Jun 13, 2025

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
11:04

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides

Published on: September 7, 2019

9.1K

使用机器学习预测电子喷雾电离质谱的化电离效率.

Justin A Kaskow1, Eric T Hahnert1, Thomas K Porter1

  • 1David H. Koch School of Chemical Engineering Practice, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.

Journal of the American Society for Mass Spectrometry
|September 9, 2024
PubMed
概括

使用机器学习预测离子化效率 (IE) 提高了无标签质谱 (MS) 量化. 这种方法提高了复杂生物样本中蛋白质量化和化物翻译后修饰分析的准确性.

更多相关视频

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
05:47

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Published on: February 19, 2020

9.4K
Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
11:54

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Published on: March 23, 2020

9.4K

相关实验视频

Last Updated: Jun 13, 2025

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
11:04

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides

Published on: September 7, 2019

9.1K
Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
05:47

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Published on: February 19, 2020

9.4K
Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
11:54

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Published on: March 23, 2020

9.4K

科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 分析化学 分析化学
  • 生物技术是生物技术.

背景情况:

  • 无标签质谱 (MS) 对于分析复杂的生物系统至关重要.
  • 在MS中精确的量化依赖于从的物理化学性质中预测电离效率 (IE).
  • 与小分子相比,对模拟IE存在有限的研究.

研究的目的:

  • 使用机器学习开发离子化效率 (IE) 的预测模型.
  • 为和蛋白质建立准确的无标签量化工作流程.
  • 改进对的翻译后修饰 (PTMs) 的量化.

主要方法:

  • 从单克隆抗体 (mAbs) 的素消化剂中编制了241个的相对电离效率 (RIEs) 的数据集.
  • 从它们的序列中计算出体物理化学描述符.
  • 训练有素的机器学习模型,包括随机森林 (RF) 和多层感知子 (MLP),以预测RIEs.

主要成果:

  • 对于小于20个氨基酸的,RIEs与分子量有很强的相关性.
  • 一个随机森林 (RF) 模型在预测RIEs时实现了23.9%的平均相对误差.
  • 一种多层感知子 (MLP) 模型将较大的的平均相对误差从60.5%降至32.0%,优于现有方法.

结论:

  • 从序列中预测IE可以实现准确的无标签量化.
  • 开发的模型增强了相对蛋白质量化和PTM分析.
  • 这种方法支持高通量蛋白质组学和生物标志物发现的进步.