相关实验视频

Updated: Jun 13, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

双向聚合-转录放大-编码双色光生物传感器,用于无标签和无原料检测多个piRNAs

Weijie Tong¹, Yun Han¹, Tao Wang²

¹School of Chemistry and Chemical Engineering, State Key Laboratory of Digital Medical Engineering, Southeast University, Nanjing 211189, China.

Analytical chemistry

|September 9, 2024

概括

我们开发了一种新的生物传感器,用于检测多个PIWI相互作用RNA (piRNAs),而无需标签或原料. 这种敏感的平台可以量化单细胞,并区分癌细胞,为诊断提供了强大的工具.

科学领域:

分子生物学分子生物学
生物化学生化学
生物技术是生物技术.

背景情况:

与PIWI相互作用的RNAs (piRNAs) 是关键的非编码RNAs,调节细胞过程.
皮RNAs的失调与包括癌症在内的各种病理状况有关.
准确和敏感的piRNA检测方法对于临床应用至关重要.

研究的目的:

开发一种新的,无标签的,无原料的生物传感器,用于对多个piRNA的敏感和特定检测.
建立一个平台,以单细胞分辨率量化细胞piRNAs.
证明生物传感器在区分癌细胞和组织方面的实用性.

主要方法:

采用了双向聚合转录放大策略.
一个针头探针作为识别单元,原料和转录模板.
使用RNA胺-胺复合体 (DFHBI-1T/MG) 进行双色光检测.

主要成果:

生物传感器实现了piRNAs的超敏感检测,检测极限低至44.44 aM.
细胞piRNAs的单细胞量化成功进行.
生物传感器有效地将癌细胞与正常细胞区分开来,并在乳腺癌组织中分化piRNA表达.

结论:

开发的生物传感器提供了一个强大而通用的平台,用于对多个piRNAs进行敏感,无标签和无原始检测.
这项技术在推进与piRNA相关的临床诊断和治疗方面具有重大潜力.
可适应的设计允许通过修改探针序列来检测各种piRNA.

更多相关视频

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Published on: October 18, 2024

相关概念视频

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...