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评估与Saccharomyces cerevisiae中的三糖降解基因相关的细胞作用和表型

Anqi Chen^1,2, Sara E Stadulis¹, Kayla deLeuze¹

¹Department of Food Science, Cornell University, Ithaca, NY 14853, USA.

G3 (Bethesda, Md.)

|September 9, 2024

概括

在野生酵母菌株中破坏三酶基因 (NTH1,NTH2,ATH1) 揭示了三酶积累和子发芽受损. 这些发现强调了三酶在应力恢复和细胞循环调节中的作用.

科学领域:

* 微生物学和分子生物学
* 酵母遗传学和生理学

背景情况:

*三糖是一种二糖,是酵母中 (*Saccharomyces cerevisiae*) 重要的能量储备和压力保护剂.
*三酶酶 (中性Nth1,Nth2;酸Ath1) 负责三糖的分解.
*以前的研究集中在实验室酵母菌株的特雷哈拉酶功能上.

研究的目的:

* 调查各种野生酵母菌株中破坏三酶基因 (NTH1,NTH2,ATH1) 的表型影响.
* 为了确定实验室观察到的三酶表型是否在野生酵母种群中保存.
*阐明中性和酸性三酶在三糖代谢和细胞过程中的特定作用.

主要方法:

* 在五种不同的*S. cerevisiae*野生菌株中构建单基和双基三酶删除突变物 (nth1Δ,nth2Δ,ath1Δ,nth1nth2Δ).
*表型分析包括三和糖原的生产,细胞大小,耐热性,高温生长,化和碳来源的利用.
*对细胞内和细胞外三糖降解能力的评估.

主要成果:

*与野生型菌株相比,所有三酶突变体都表现出细胞内三糖积累的增加.
* 缺乏中性三酶活性 (nth1Δ,nth1nth2Δ) 的突变体表现出轻微的热敏感性,这表明它们在应激恢复中发挥了作用.
*细胞内三糖水解对于子正常发芽至关重要,这表明它在细胞循环调节中的作用.
* Ath1被证实是用于利用细胞外三糖作为碳来源的必不可少的.

结论:

*三酶基因的破坏导致各种野生酵母菌株的三糖积累.
* 中性特雷哈拉酶在酵母耐热性和应激反应中起作用.
* 细胞内三糖水解对于酵母子发芽和细胞周期进展至关重要.
* Ath1对于利用外部三糖是必不可少的,独立于其他传送器,如AGT1.

关键词:

在ATH1中使用ATH1.在NTH1中,NTH1是NTH1.在NTH2中,NTH2是NTH2.这种植物是Saccharomyces cerevisiae.遗传异质性的遗传异质性三化物三化物三醇降解降解过程

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