生物发光共振能量转移 (BRET) - 自发光光感应生物材料中蛋白质释放的介导
Teresa L Rapp1, Irina Kopyeva2, Abhinav Adhikari1
1Department of Chemical Engineering, University of Washington, Seattle, Washington 98105, United States.
Journal of the American Chemical Society
|September 9, 2024
概括
我们开发了一种新的光激活药物递送系统,使用生物发光共振能量转移 (BRET) 进行精确的治疗释放. 这种方法克服了光透的极限,在全身注射后可实现局部药物输送.
科学领域:
- 生物材料科学 生物材料科学
- 药物输送系统 药物输送系统
- 生物技术是生物技术.
背景情况:
- 光触发释放为治疗应用提供时空控制.
- 有限的光透限制了当前的光动力学疗法在表面组织.
- 需要新的策略来实现深层组织或全身光触发药物释放.
研究的目的:
- 开发一种使用生物发光共振能量转移 (BRET) 从水凝释放光触发生物分子的新方法.
- 为了克服光动力学治疗释放中光透深度的局限性.
- 为了使局部药物输送系统管理后.
主要方法:
- 合成一种异性双功能交叉链接器 (CHO-Ru-N3) 用于对光敏感的水凝功能化.
- 纳诺化酶 (NLuc) 与以聚乙烯糖醇 (PEG) 为基础的水凝的结合,通过排列酶标记增强蛋白质结合 (STEPL) 和应变促进的亚酸环添加 (SPAAC).
- 货物的释放 (表皮生长因子,EGF) 的演示,由直接光或BRET介导的生物光解触发.
主要成果:
- 实现了光敏交叉连接器的简单,高产合成.
- 证明了成功的光诱导联结体交换和水凝功能化.
- 通过直接照明和基于BRET的生物发光溶解,实现了EGF的快速和同等释放.
- 纳米化酶发光允许可控制的能量转移用于治疗释放.
结论:
- 基于BRET的生物发光溶解为直接光线提供了一个可行的替代方案,用于触发水凝释放货物.
- 这一策略有效地克服了传统光动力学疗法的透深度限制.
- 开发的系统对本地化药物输送应用有前途,特别是在系统管理后.
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