在不同的盐度中,TPT破坏了海洋medaka的早期胚胎发育和葡萄糖代谢
Tengzhou Li1, Luoxin Li2, Bin Liu1
1Marine College, Shandong University, Weihai, Shandong 264209, China.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
|September 9, 2024
概括
暴露于三烯 (TPT) 增加了海洋医学和胚胎死亡率,特别是在低盐度和高盐度的水域. TPT 破坏葡萄糖代谢和胰岛素信号通路,影响早期发育.
科学领域:
- 环境毒理学环境毒理学
- 海洋生物学 海洋生物学
- 生态毒理学 生态毒理学
背景情况:
- 有机蛋白化合物,特别是三烯 (TPT),在沿海河口普遍存在.
- 关于TPT在盐度波动的河口环境中的毒理作用的研究有限.
研究的目的:
- 在不同的盐度条件下,研究TPT对海洋 (Oryzias melastigma) 胚胎的毒性影响.
- 探索TPT对早期胚胎发育的影响,重点关注代谢途径和基因表达.
主要方法:
- 在不同的盐度 (0 ppt和30 ppt) 中,海洋medaka胚胎暴露在TPT中.
- 评估胚胎死亡率,进行RNA测序 (RNA-seq) 进行基因表达分析,并使用生物化学分析和qPCR.
主要成果:
- 在0 ppt和30 ppt盐度下,TPT暴露显著增加了胚胎死亡率.
- RNA-seq数据表明TPT主要影响葡萄糖代谢和糖原合成,可能抑制糖解并促进高盐度下的葡萄糖生成.
- 暴露于TPT下调了胰岛素信号通路中的关键基因 (ins,insra,irs2b,pik3ca,pdk1b,akt1,foxo1a).
结论:
- 暴露于TPT会对不同盐度的海洋医学和早期胚胎发育产生负面影响.
- TPT抑制葡萄糖代谢和胰岛素信号通路,导致河口环境中的胚胎死亡率增加.
- 这些发现为了解沿海生态系统中的有机化合物毒性提供了有价值的数据.
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