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在出生后发育期间,小鼠心脏中的线素激活蛋白激酶信号:MAPKs,MAP3Ks,MAP4Ks和DUSPs

Hajed O Alharbi¹, Peter H Sugden², Angela Clerk²

¹Department of Medical Laboratory, College of Applied Medical Sciences, Quassim University, Buraydah, Saudi Arabia; School of Biological Sciences, University of Reading, Reading, UK.

Cellular signalling

|September 9, 2024

概括

在老鼠中,产后心脏生长涉及心肌细胞大小的增加,而不是分裂. 在此期间,基激活蛋白激酶 (MAPK) 及其调节剂的表达差异化,这表明它在心脏重塑中发挥了作用.

科学领域:

心血管生物学心血管生物学
分子和细胞生物学分子和细胞生物学
发展生物学发展生物学

背景情况:

哺乳动物心肌细胞在产前分化,在出生后停止细胞动力学.
动物心肌细胞主要通过在产后增加细胞大小来生长,成为双核细胞.
正在研究调节心肌细胞生长的机制以及信号通路的作用.

研究的目的:

为了研究 mitogen-activated protein kinases (MAPKs) 和它们相关的调节蛋白在产后大鼠心脏发育过程中的调节.
了解MAPKs在心肌细胞生长和出生后心脏重塑中的作用.

主要方法:

评估了线粒分裂/细胞动化和DNA复制标记的基因表达.
通过免疫栓塞量化总和化 (激活) MAPKs (ERK1/2,JNKs,p38).
分析了MAPK调节激酶 (MAP3Ks,MAP4Ks) 和酸酶的表达.

主要成果:

在产后观察到的线粒分裂/细胞动化基因的显著下调和DNA复制基因的较小下调.
在产后发育期间,ERK1/2,JNK和p38-MAPK的相对酸化增加.
对MAPK通路组件的差异调节,一些MAP3Ks/MAP4Ks在人类心脏衰竭中被调高.

结论:

在产后心脏发育和重塑过程中,MAPK信号通路受到动态调节.
控制MAPK的酶和酶也具有差异性表达,这意味着它们与心脏生长有关.
在人类心力衰竭中,特定的MAP3Ks/MAP4Ks的上调表明它在与疾病相关的心脏重塑中起着作用.

关键词:

心脏肌细胞是心脏肌细胞.基因表达基因表达基因表达心灵的心脏心脏的心脏心脏心脏心脏衰竭是因为心脏衰竭.细胞内信号传递是细胞内信号传递.产后发展产后发展

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MAPK Signaling Cascades

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Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...