pyrifos-oxon诱导的神经元细胞死亡通过内 плазма网膜压力触发的亡途径
Baihuan Feng1, Jingchun Lu2, Wei Jiang3
1Department of Infection Prevention and Control, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310016, China; Institute of Environmental Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.
概括
pyrifos-oxon通过诱导内质网膜应激和反应性氧物种来触发神经元细胞死亡. 针对这些途径可能为与有机酸盐相关的神经退行性疾病提供新的治疗方法.
科学领域:
- 神经科学是一个神经科学.
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
背景情况:
- pyrifos (CPF) 是一种广泛使用的有机农药.
- 流行病学研究将CPF暴露与神经系统疾病联系起来,但机制尚不清楚.
研究的目的:
- 研究一种有毒的CPF代谢产物 - - pyrifos-oxon (CPO) 对神经元亡的影响.
- 在SH-SY5Y细胞中探索内质网膜 (ER) 应激在CPO诱导的神经毒性的作用.
主要方法:
- 暴露SH-SY5Y细胞对CPO,并测量了亡和ER压力标志物.
- 使用了ER压力抑制剂 (4-PBA) 和IRE1α抑制剂 (STF-083010).
- 评估了活性氧物种 (ROS) 的产生和抗氧化剂 (NAC) 的影响.
主要成果:
- CPO诱导的剂量依赖性亡和ER压力蛋白表达.
- 4-PBA和STF-083010抑制了CPO诱导的亡和ER压力.
- CPO增加了ROS生成;NAC部分降低了细胞亡,但没有激活IRE1α.
- 结合NAC和STF-083010有效地阻止了CPO诱导的亡.
结论:
- 通过ER压力介导的IRE1α/XBP1信号传递和ROS生成,CPO诱导神经细胞毒性.
- ER压力在CPF诱导的神经毒性中发挥着关键作用.
- 针对ER压力路径为与有机酸盐相关的神经退行性疾病提供了潜在的治疗策略.
关键词:
基基酸盐 (Chlorpyrifos) 是一种基酸盐.pyrifos-oxon-oxon-oxon-oxon-oxon-oxon-oxon pyrifos-oxon-oxon pyrifos-oxon-oxon pyrifos-oxon-oxon pyrifos-oxon-oxon pyrifos-oxon pyrifos-oxon pyrifos-oxon pyrifos-oxon pyrifos-oxon pyrifos-oxon pyrifos-oxon pyrifos-oxon pyrifos-oxon pyrifos-oxon pyrifos-oxon pyrifos-oxon pyrifos-oxon pyrifos-oxon pyrifos-oxon pyrifos-oxon pyrifos-oxon pyrifos-oxon pyrifos-oxon 是 是 是 是 是 是 是 是 是 是 是细胞内膜网膜应激压力神经毒性 神经毒性有反应性氧物种的反应性氧物种.
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