氧化和酸通过改善植物化物代谢和表观遗传修饰DNA甲基化,赋予 (Cd) 耐受性
Fatemeh Farahani1, Alireza Iranbakhsh1, Mostafa Ebadi2
1Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Environmental pollution (Barking, Essex : 1987)
|September 9, 2024
概括
氧化 (NO) 和酸 (Asc) 通过表观遗传修改DNA并增强保护性化合物来增强红枣中的 (Cd) 耐受性. 这些治疗方法改善了植物对重金属毒性的防御机制.
科学领域:
- 植物科学 植物科学
- 环境毒理学环境毒理学
- 分子生物学分子生物学
背景情况:
- (Cd) 污染对植物健康和农业生产力构成重大威胁.
- 了解植物对重金属压力的反应对于开发有效的补救策略至关重要.
- 红枣 (Zataria multiflora) 是一种有价值的药用植物,容易受到重金属毒性的影响.
研究的目的:
- 调查氧化 (NO) 和酸 (Asc) 对Zataria多种植物中 (Cd) 毒性的保护作用.
- 阐明NO和Asc.所赋予的Cd耐受性的基础分子和生物化学机制.
- 评估NO和Asc对DNA甲基化,基因表达和Cd压力的代谢物积累的影响.
主要方法:
- 红枣苗被单独用 (Cd) 或与氧化 (NO) 或酸 (Asc) 结合处理.
- 评估了射击生物量,DNA甲基化模式 (低甲基化和半甲基化) 和基因表达 (γ-特尔皮宁合成酶,P450单氧化酶).
- 量化关键代谢物 (proline,carvacrol,thymol,flavonoids) 和评估的蛋白质含量,氨酸氨基酶 (PAL) 活性和抗氧化酶活性 (catalase,过氧化酶).
主要成果:
- 暴露于Cd减少了树苗的生长生物质,这种生物质通过NO和Asc处理来减轻.
- NO和Asc显著诱导DNA低甲基化和增加非甲基化百分比.
- 在NO和Asc治疗时观察到TPS,CYP71D178和CYP71D180基因的升调.
- 增加了proline,carvacrol,thymol和flavonoids的积累,也可以观察到.
- NO和Asc治疗恢复了蛋白质含量,增加了PAL活性,并增强了抗氧化酶活性.
结论:
- 氧化 (NO) 和酸 (Asc) 在Zataria多种植物中有效地赋予 (Cd) 耐受性.
- 通过DNA低甲基化进行表观遗传改造是Cd耐受性的关键机制.
- NO和Asc调节了类和类代谢,增强了类的积累,并改善了抗氧化剂防御系统.
- 这些发现为减轻植物中的重金属压力提供了新的见解,通过对外应用NO和Asc.
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