在卵巢成熟过程中,Neocaridina denticulata sinensis中的两个Gustavus拼接变体的功能补充
Meiling Liang1,2, Dandan Feng1, Jiquan Zhang3
1School of Life Sciences/Hebei Basic Science Center for Biotic Interaction, Hebei University, Baoding, 071002, China.
Scientific reports
|September 9, 2024
概括
古斯塔弗斯 (Gustavus) 蛋白质 (SPSB) 调节了的繁殖. 两个异构体,NdGusX1和NdGusX2,对于卵巢成熟和生产生至关重要,影响卵子的发育.
科学领域:
- 甲动物生物学 甲动物生物学
- 分子遗传学 分子遗传学
- 生殖生物学 生殖生物学
背景情况:
- 古斯塔弗斯蛋白质是SPSB家族的一部分,对于关节动物的繁殖至关重要.
- 该SPSB家族 (SPSB1-4) 影响关键过程,如免疫力,亡,生长和压力反应在更高的动物.
研究的目的:
- 研究两个Neocaridina denticulata sinensis Gustavus异型 (NdGusX1和NdGusX2) 在卵巢成熟中的作用.
- 探索这些异构体在繁殖中的表达模式和功能意义.
主要方法:
- 对替代拼接进行分析,以识别NdGusX1和NdGusX2异型.
- 定量表达分析 (包括现场杂交) 和RNA干扰 (RNAi).
- 在基因淘汰后评估维特洛根因合成和卵细胞发育.
主要成果:
- NdGusX1和NdGusX2表现出明显的组织表达 (卵巢和肌肉),并且存在于所有卵巢生殖细胞中.
- 这两种异构体都因铜离子暴露而升级,并在卵细胞中表现出特定的表达模式.
- RNAi knockdown 干扰了维特洛因的合成,影响了维特洛因的产生和卵细胞大小,表明在卵巢成熟过程中的功能性作用.
结论:
- NdGustavus异型在甲类卵巢成熟和生过程中发挥着至关重要的作用.
- 卵巢和肌肉表达之间存在功能补充和差异化.
- 这项研究提供了关于SPSB家族在繁殖过程中的作用的见解.
相关概念视频
Complementation Tests
4.9K
A complementation test is a simple cross to identify whether the two mutations are located on the same gene or different genes. It was first performed by Edward Lewis in the 1940s while working on fruit flies. He developed the test to identify the location and arrangement of different mutations on chromosomes.
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...
4.9K
Alternative RNA Splicing
21.0K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.0K
RNA Splicing
56.2K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.2K
Dosage Compensation
6.1K
In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...
6.1K
Exon Recombination
3.6K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.6K
Genetics of Speciation
19.1K
Speciation is the evolutionary process resulting in the formation of new, distinct species—groups of reproductively isolated populations.
19.1K


